...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Microbiology and Biotechnology >Antifungal mechanism of an anti-Pythium protein (SAP) from the marine bacterium Streptomyces sp strain AP77 is specific for Pythium porphyrae, a causative agent of red rot disease in Porphyra spp.
【24h】

Antifungal mechanism of an anti-Pythium protein (SAP) from the marine bacterium Streptomyces sp strain AP77 is specific for Pythium porphyrae, a causative agent of red rot disease in Porphyra spp.

机译：海洋细菌链霉菌属菌株AP77的抗腐霉菌蛋白（SAP）的抗真菌机制对腐霉菌（Pythium porphyrae）是特有的，腐霉菌是红斑病的致病菌。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Previously we reported an antifungal protein specific to Pythium porphyrae, a causative agent of red rot disease afflicting seaweed Porphyra spp. This study was carried out to identify the antifungal mechanism of the antifungal protein to P. porphyrae. When we first examined the effect of an anti-Pythium protein (SAP) on the P. porphyrae cell walls, SAP did not decompose the six structural polysaccharides in Pythium cell walls. However, hyphal growth was significantly inhibited in Pythium cells treated with 50 mug/ml of SAP by MTT assay. Protoplasmic leakage was observed in P. porphyrae hyphae treated with SAP for I h, followed by hyphal swelling and disintegration, using SYTOX Green, and SAP permeabilized the membrane of P. porphyrae in a dose-dependent manner. Treating P. porphyrae cells with SAP in the presence of carbonyl cyanide m-chlorophenylhydrazone (CCCP), a membrane-depolarizing agent, significantly reduced the membrane permeability to SYTOX Green. Moreover, a similar effect was observed when the P. porphyrae cells were treated with SAP in the presence Of MgCl2. In contrast, identical treatment in the presence of KCl significantly increased the membrane permeability to SYTOX Green. These results suggested that anti-Pythium mechanism of SAP was related to alteration of the membrane permeability in P. porphyrae. [References: 30]
机译：以前，我们报道了一种对紫菜腐霉具有特异性的抗真菌蛋白，该腐霉病是困扰紫菜紫菜的红腐病的病原体。进行了这项研究以鉴定抗真菌蛋白对卟啉单胞菌的抗真菌机理。当我们首次检查抗腐霉菌蛋白（SAP）对卟啉单胞菌细胞壁的影响时，SAP并没有分解腐霉菌细胞壁中的6种结构多糖。然而，通过MTT分析，在用50杯/毫升的SAP处理的腐霉属细胞中，菌丝的生长被显着抑制。用SYTOX Green在SAP处理过1 h的卟啉菌菌丝中观察到原生质渗漏，然后使用SYTOX Green进行菌丝肿胀和崩解，SAP以剂量依赖的方式渗透了卟啉菌的膜。在膜去极化剂羰基氰化物间氯苯hydr（CCCP）的存在下，用SAP处理卟啉单胞菌细胞显着降低了膜对SYTOX Green的渗透性。此外，当在MgCl 2存在下用SAP处理卟啉单胞菌细胞时，观察到类似的效果。相反，在氯化钾存在下的相同处理显着提高了膜对SYTOX Green的渗透性。这些结果表明SAP的抗腐霉菌机制与紫菜的膜通透性的变化有关。 [参考：30]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号