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首页> 外文期刊>Applied Microbiology and Biotechnology >A whole cell biocatalyst for cellulosic ethanol production from dilute acid-pretreated corn stover hydrolyzates
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A whole cell biocatalyst for cellulosic ethanol production from dilute acid-pretreated corn stover hydrolyzates

机译：一种全细胞生物催化剂，用于由稀酸预处理的玉米秸秆水解产物生产纤维素乙醇

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In this research, a recombinant whole cell biocatalyst was developed by expressing three cellulases from Clostridium cellulolyticum-endoglucanase (Cel5A), exoglucanase (Cel9E), and β-glucosidase-on the surface of the Escherichia coli LY01. The modified strain is identified as LY01/pRE1H-AEB. The cellulases were displayed on the surface of the cell by fusing with an anchor protein, PgsA. The developed whole cell biocatalyst was used for single-step ethanol fermentation using the phosphoric acid-swollen cellulose (PASC) and the dilute acid-pretreated corn stover. Ethanol production was 3.59±0.15 g/L using 10 g/L of PASC, which corresponds to a theoretical yield of 95.4±0.15%. Ethanol production was 0.30±0.02 g/L when 1 g/L equivalent of glucose in the cellulosic fraction of the dilute sulfuric acid-pretreated corn stover (PCS) was fermented for 84 h. A total of 0.71±0.12 g/L ethanol was produced in 48 h when the PCS was fermented in the simultaneous saccharification and co-fermentation mode using the hemicellulosic (1 g/L of total soluble sugar) and as well as the cellulosic (1 g/L of glucose equivalent) parts of PCS. In a control experiment, 0.48 g/L ethanol was obtained from 1 g/L of hemicellulosic PCS. It was concluded that the whole cell biocatalyst could convert both cellulosic and hemicellulosic substrates into ethanol in a single reactor. The developed C. cellulolyticum-E. coli whole cell biocatalyst also overcame the incompatible temperature problem of the frequently reported fungal-yeast systems.
机译：在这项研究中，通过在大肠杆菌LY01的表面上表达来自解纤梭菌-内葡聚糖酶（Cel5A），外切葡聚糖酶（Cel9E）和β-葡萄糖苷酶的三种纤维素酶，开发了一种重组全细胞生物催化剂。修饰的菌株被鉴定为LY01 / pRE1H-AEB。通过与锚蛋白PgsA融合，将纤维素酶展示在细胞表面。使用磷酸溶胀的纤维素（PASC）和稀酸预处理的玉米秸秆，将开发的全细胞生物催化剂用于一步乙醇发酵。使用10 g / L的PASC，乙醇产量为3.59±0.15 g / L，这对应于95.4±0.15％的理论收率。当将稀硫酸预处理的玉米秸秆（PCS）的纤维素级分中的葡萄糖当量1 g / L发酵84小时时，乙醇产量为0.30±0.02 g / L。当使用半纤维素（1 g / L的总可溶性糖）和纤维素（1）同时糖化和共同发酵模式进行PCS发酵时，在48小时内共产生0.71±0.12 g / L乙醇。 g / L葡萄糖当量）部分。在对照实验中，从1 g / L的半纤维素PCS中获得了0.48 g / L的乙醇。结论是，全细胞生物催化剂可以在单个反应器中将纤维素和半纤维素底物转化为乙醇。发达的解纤梭菌E。大肠杆菌全细胞生物催化剂还克服了经常报道的真菌-酵母系统不兼容的温度问题。

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