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首页> 外文期刊>Applied Microbiology and Biotechnology >Prolongation of electrode lifetime in biofuel cells by periodic enzyme renewal
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Prolongation of electrode lifetime in biofuel cells by periodic enzyme renewal

机译：通过定期更新酶来延长生物燃料电池中的电极寿命

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Enzymatically catalyzed biofuel cells show unique specificity and promise high power densities, but suffer from a limited lifetime due to enzyme deactivation. In the present work, we demonstrate a novel concept to extend the lifetime of a laccase-catalyzed oxygen reduction cathode in which we decouple the electrode lifetime from the limited enzyme lifetime by a regular resupply of fresh enzymes. Thereto, the adsorption behavior of laccase from Trametes versicolor to buckypaper electrode material, as well as its time-dependent deactivation characteristics, has been investigated. Laccase shows a Langmuir-type adsorption to the carbon nanotube-based buckypaper electrodes, with a mean residence time of 2 days per molecule. In a citrate buffer of pH 5, laccase does not show any deactivation at room temperature for 2 days and exhibits a half-life of 9 days. In a long-term experiment, the laccase electrodes were operated at a constant galvanostatic load. The laccase-containing catholyte was periodically exchanged against a freshly prepared one every second day to provide sufficient active enzymes in the catholyte for the replacement of desorbed inactive enzymes. Compared to a corresponding control experiment without catholyte exchange, this procedure resulted in a 2.5 times longer cathode lifetime of 19±9 days in which the electrode showed a potential above 0.744 V vs. normal hydrogen electrode at 110 μAcm-2. This clearly indicates the successful exchange of molecules by desorption and re-adsorption and is a first step toward the realization of a self-regenerating enzymatic biofuel cell in which enzyme-producing microorganisms are integrated into the electrode to continuously resupply fresh enzymes.
机译：酶催化的生物燃料电池显示出独特的特异性并有望实现高功率密度，但由于酶失活而寿命有限。在当前的工作中，我们展示了一种新颖的概念，可延长漆酶催化的氧还原阴极的寿命，其中我们通过定期补充新鲜的酶，将电极寿命与有限的酶寿命脱钩。迄今为止，已经研究了漆酶从杂色Trametes到巴基纸电极材料的吸附行为，以及其随时间变化的失活特性。漆酶对碳纳米管基布基纸电极表现出Langmuir型吸附，每分子平均停留时间为2天。在pH 5的柠檬酸盐缓冲液中，漆酶在室温下2天没有任何失活，并且半衰期为9天。在长期实验中，漆酶电极在恒定的恒电流负载下运行。每隔一天将含漆酶的阴极液定期更换为新鲜制备的一种，以在阴极液中提供足够的活性酶，以替代解吸的惰性酶。与没有进行阴极电解液交换的相应对照实验相比，此程序导致阴极寿命（19±9天）延长了2.5倍，其中在110μAcm-2下，电极显示出比普通氢电极高0.744 V的电势。这清楚地表明了通过解吸和再吸附成功进行了分子交换，这是实现自再生酶促生物燃料电池的第一步，其中将产生酶的微生物整合到电极中以连续地重新供应新鲜的酶。

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