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首页> 外文期刊>Applied Microbiology and Biotechnology >Induction of hexanol dehydrogenase in Geotrichum spp. by the addition of hexanol
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Induction of hexanol dehydrogenase in Geotrichum spp. by the addition of hexanol

机译：Geotrichum spp中己醇脱氢酶的诱导。通过添加己醇

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摘要

Excessive hexanol content distorts the flavor of foods and is harmful to human health. Previously, two strains of fungi were found capable of producing hexanol-degrading enzymes. The current study identified these strains as Galactomyces geotrichum according to the gene sequence of the 26 S rDNA D1/D2 region (strain S12) and genus Geotrichum according to the gene sequence of ITS region (strain S13). Parallel analysis of extracellular and intracellular enzyme activities showed that the enzymes mainly accumulated intracellularly. Native polyacrylamide gel electrophoresis with reactive dyes showed the enzymes were alcohol dehydrogenases induced by the addition of hexanol. Hexanol was catalyzed into hexanoic acid and hexanal by strain S12 and into hexanoic acid by strain S13. The optimum conditions for the induction of enzymes were determined to be 6-9 h in the presence of 0.7 g/l hexanol. The identification of two strains capable of enzymatically degrading hexanol and optimum conditions for their induction will facilitate their use in industrial applications.
机译：过多的己醇含量会扭曲食品的味道，并且对人体健康有害。以前，发现了两种能够产生己醇降解酶的真菌。目前的研究根据26 S rDNA D1 / D2区的基因序列（菌株S12）将这些菌株鉴定为Geogrichrichum，根据ITS区的基因序列将菌株Geotrichum鉴定为Geotrichum（菌株S13）。细胞外和细胞内酶活性的平行分析表明，这些酶主要在细胞内积累。用活性染料进行的天然聚丙烯酰胺凝胶电泳显示，酶是通过添加己醇诱导的醇脱氢酶。己醇被菌株S12催化生成己酸和己醛，并被菌株S13催化生成己酸。在0.7 g / l的己醇存在下，确定诱导酶的最佳条件为6-9 h。鉴定两种能够酶促降解己醇的菌株及其诱导的最佳条件将有助于它们在工业应用中的使用。

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