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首页> 外文期刊>Applied Microbiology and Biotechnology >Enhancement of Taxol production and excretion in Taxus chinensis cell culture by fungal elicitation and medium renewal
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Enhancement of Taxol production and excretion in Taxus chinensis cell culture by fungal elicitation and medium renewal

机译：真菌诱导和培养基更新促进红豆杉细胞培养中紫杉醇的产生和排泄

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An endophytic fungus, Aspergillus niger, isolated from the inner bark of a Taxus chinensis tree, was used as an elicitor to stimulate the Taxol (paclitaxel) production in a Taxus chinensis cell suspension culture. Different elicitor doses and elicitation times were tested in a batch culture: and the highest volumetric Taxol yield was achieved when 40 mg of the fungal elicitor (carbohydrate equivalent) l(-1)' was added to the culture during the late exponential-growth phase. The elicitation resulted in a more than two-fold increase in the Taxol yield and about a six-fold increase in total secretion. The Taxol yield was further improved substantially by applying medium renewal and re-elicitation to the culture. In particular, with repeated medium renewal (in a way similar to medium perfusion) and a second elicitation of the culture, the volumetric Taxol yield was increased to 67.1 +/-7.5 mg l(-1), which was about seven times the amount obtained in the non-elicited batch culture. The Taxol productivity of the perfusion-like culture with repeated fungal elicitation was 1.5 mg l(-1) day(-1), which was about 40% higher than that of the elicitor-treated batch culture and three times the productivity of the non-elicited batch culture. [References: 24]
机译：从红豆杉树的内部树皮中分离出的内生真菌黑曲霉被用作激发剂，以刺激红豆杉细胞悬浮培养物中紫杉醇（紫杉醇）的产生。在分批培养中测试了不同的激发剂剂量和激发时间：当在指数增长后期将40 mg的真菌激发剂（碳水化合物当量）l（-1）'添加到培养物中时，紫杉醇的最高体积产量达到了最高。 。诱发导致紫杉醇产量增加两倍以上，总分泌物增加约六倍。通过对培养基进行培养基更新和重新诱导，紫杉醇的产量进一步得到了大大提高。特别是，通过反复进行培养基更新（类似于培养基灌注）和第二次诱导培养，紫杉醇的体积产量增加到了67.1 +/- 7.5 mg l（-1），约为其量的七倍。在非分批培养中获得。反复真菌诱导的灌注样培养物的紫杉醇生产率为1.5 mg l（-1）天（-1），比诱导剂处理的分批培养物高约40％，是非诱导培养物的三倍。引发的分批培养。 [参考：24]

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