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首页> 外文期刊>Applied Microbiology and Biotechnology >Microbial community changes during organic solid waste treatment analyzed by double gradient-denaturing gradient gel electrophoresis and fluorescence in situ hybridization
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Microbial community changes during organic solid waste treatment analyzed by double gradient-denaturing gradient gel electrophoresis and fluorescence in situ hybridization

机译：通过双梯度变性梯度凝胶电泳和荧光原位杂交分析有机固体废物处理过程中的微生物群落变化

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摘要

The bacterial community present during semicontinuous treatment of organic solid waste under alkaline and high-temperature conditions was studied. PCR-amplified 16S rDNA fragments were analyzed by double gradient-denaturing gradient gel electrophoresis (DGGE). The band pattern was stable during the steady state of the treatment phase, and the major bands resulting from individual treatments had the same DNA sequence with good reproducibility. No sequence in the DNA database of isolated bacteria showed close similarity to this sequence, the closest relative being Bacillus licheniformis with less than 97% similarity. The conditions for fluorescence in situ hybridization (FISH) were determined without the need to obtain extracts of the bacterial cells. An oligonucleotide probe was designed to detect the microorganisms found in the DGGE analysis. FISH analysis showed that the bacterium corresponding to the major bands accounted for 30% of the total eubacterial cell count at the steady state. These results indicate that this bacterium is a key microorganism in the biodegradation process. [References: 33]
机译：研究了在碱性和高温条件下半连续处理有机固体废物过程中存在的细菌群落。通过双梯度变性梯度凝胶电泳（DGGE）分析PCR扩增的16S rDNA片段。在治疗阶段的稳定状态下，条带模式是稳定的，并且各个处理产生的主要条带具有相同的DNA序列，并具有良好的可重复性。在分离的细菌的DNA数据库中没有序列显示出与该序列的相似性，最接近的亲戚是地衣芽孢杆菌，其相似性小于97％。无需获得细菌细胞提取物即可确定荧光原位杂交（FISH）的条件。设计寡核苷酸探针以检测在DGGE分析中发现的微生物。 FISH分析表明，在稳定状态下，对应于主要谱带的细菌占总真细菌细胞数的30％。这些结果表明该细菌是生物降解过程中的关键微生物。 [参考：33]

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