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首页> 外文期刊>Applied Microbiology and Biotechnology >A microbial method using whole cells of Thiobacillus thiooxidans for measuring sulphate in waters
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A microbial method using whole cells of Thiobacillus thiooxidans for measuring sulphate in waters

机译：一种使用硫代硫氧硫杆菌全细胞测定水中硫酸盐的微生物方法

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摘要

A microbial method to determine sulphate concentration in water was developed on the basis of sulphate-dependent acid phosphatase (APase) in whole cells of Thiobacillus thiooxidans. The activity of the APase was determined colorimetrically by using p-nitrophenylphosphate as substrate. The APase was activated by sulphate. A linear relationship was obtained between the activity of the APase and the concentration of sulphate in the range 0-0.6 mM. Therefore, the sulphate concentration was estimated from the APase activity, represented by the absorbance (A(400)) The microbial method was applied to the determination sulphate in water. The lower limit of detection was 0.02 mM, the relative standard deviation being 2% for 10 measurements on a standard sample. As for practical samples, which were taken from rain, river and tap water, good agreement was obtained between the values measured by the microbial method and those given by a conventional barium chloranilate method. The relative standard deviation was 2.1% for 12 measurements of tap water. The activity of the APase was stable over a period of more than 100 days when the cells were stored in 0.1 M sodium acetate/acetic acid buffer (pH 5.0) at 4 degrees C.
机译：基于硫代氧化硫硫杆菌的全细胞中硫酸盐依赖性酸性磷酸酶（APase），开发了一种测定水中硫酸盐浓度的微生物方法。以对硝基苯基磷酸酯为底物，通过比色法测定APase的活性。 APase被硫酸盐激活。在APase的活性和0-0.6 mM范围内的硫酸盐浓度之间获得了线性关系。因此，硫酸盐浓度由APase活性估算，以吸光度（A（400））表示。微生物方法用于测定水中的硫酸盐。检测下限为0.02 mM，对标准样品进行10次测量的相对标准偏差为2％。对于从雨水，河水和自来水采集的实际样品，通过微生物方法测得的值与通过常规氯苯甲酸钡方法给出的值之间取得了很好的一致性。 12次自来水的相对标准偏差为2.1％。当将细胞保存在4°C的0.1 M乙酸钠/乙酸缓冲液（pH 5.0）中时，APase的活性在超过100天的时间内稳定。

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