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首页> 外文期刊>Applied Microbiology and Biotechnology >Diversity and dynamics of dominant and rare bacterial taxa in replicate sequencing batch reactors operated under different solids retention time
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Diversity and dynamics of dominant and rare bacterial taxa in replicate sequencing batch reactors operated under different solids retention time

机译：在不同固体停留时间下运行的重复测序批量反应器中优势菌群和稀有菌群的多样性和动力学

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摘要

In this study, 16S rRNA gene pyrosequencing was applied in order to provide a better insight on the diversity and dynamics of total, dominant, and rare bacterial taxa in replicate lab-scale sequencing batch reactors (SBRs) operated at different solids retention time (SRT). Rank-abundance curves showed few dominant operational taxonomic units (OTUs) and a long tail of rare OTUs in all reactors. Results revealed that there was no detectable effect of SRT (2 vs. 10 days) on Shannon diversity index and OTU richness of both dominant and rare taxa. Nonmetric multidimensional scaling analysis showed that the total, dominant, and rare bacterial taxa were highly dynamic during the entire period of stable reactor performance. Also, the rare taxa were more dynamic than the dominant taxa despite expected low invasion rates because of the use of sterile synthetic media.
机译：在这项研究中，使用16S rRNA基因焦磷酸测序技术可以更好地了解在不同固相保留时间（SRT）下运行的重复实验室规模测序批量反应器（SBR）中总细菌，优势细菌和稀有细菌类群的多样性和动态。 ）。等级-丰度曲线显示，在所有反应堆中，主要的操作分类单位（OTU）很少，而稀有的OTU却长尾巴。结果显示，SRT（2天比10天）对优势和稀有类群的Shannon多样性指数和OTU丰富度没有可检测的影响。非度量多维标度分析表明，在整个反应堆性能稳定的整个过程中，总细菌，优势细菌和稀有细菌类群都是高度动态的。此外，尽管由于使用无菌合成培养基而预期的低入侵率，但稀有分类群比显性分类群更具活力。

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