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Growth and amino acid requirements of hyaluronic-acid-producing Streptococcus zooepidemicus

机译:产生透明质酸的兽疫链球菌的生长和氨基酸需求

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摘要

A chemically defined medium has been developed for anaerobic cultivation of hyaluronic-acid(HA)-producing Streptococcus zooepidemicus, which contains 11 amino acids essential to growth, and glutamine as a principal nitrogen source. The final HA concentration, the specific rate of HA production and HA-to-glucose yields were similar for growth in the chemically defined medium relative to growth in complex medium. Consequently cells cultivated on chemically defined medium can be expected to have similar activity regarding HA synthesis as compared to cells grown on complex media. However, the specific growth rate and volumetric HA production rate were found to be less favourable in the chemically defined media.
机译:已开发出一种化学成分确定的培养基,用于厌氧培养生产透明质酸(HA)的兽疫链球菌,其中含有11个对生长至关重要的氨基酸,并将谷氨酰胺作为主要的氮源。相对于复杂培养基中的生长,化学定义培养基中的最终HA浓度,HA产生的比速率和HA-葡萄糖产量相似。因此,与在复杂培养基上生长的细胞相比,可以预期在化学成分确定的培养基上培养的细胞在HA合成方面具有相似的活性。然而,在化学确定的培养基中,发现特定的生长速率和体积HA产生速率较差。

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