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The influence of nitrogen sources on the alpha-amylase productivity of Aspergillus oryzae in continuous cultures

机译:氮源对连续培养中米曲霉α-淀粉酶生产力的影响

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摘要

The influence of the nitrogen source on the cc-amylase productivity of Aspergillus oryzae was quantified in continuous cultivations. Both inorganic and complex nitrogen sources were investigated and glucose was used as the carbon and energy sources. For production of alpha-amylase, nitrate was shown to be inferior to ammonia as a nitrogen source. A mixture of ammonia and complex nitrogen sources, such as yeast extract or casein hydrolysate, was better than with ammonia as the sole nitrogen source. Even a low concentration of casein hydrolysate (0.05 g l(-1)) resulted in a 35% increase in the cc-amylase productivity. The higher alpha-amylase productivity during growth on casein hydrolysate was not caused by increased transcription of the alpha-amylase genes but was caused by a faster secretion of alpha-amylase or by a lower binding of alpha-amylase to the biomass. [References: 12]
机译:在连续培养中定量了氮源对米曲霉α-淀粉酶生产率的影响。研究了无机氮源和复合氮源,并使用葡萄糖作为碳源和能源。对于生产α-淀粉酶,硝酸盐被证明不如氨作为氮源。氨和复杂氮源(例如酵母提取物或酪蛋白水解物)的混合物要好于以氨为唯一氮源。甚至低浓度的酪蛋白水解物(0.05 g l(-1))也会导致c-淀粉酶生产率提高35%。在酪蛋白水解产物上生长期间较高的α-淀粉酶生产率不是由α-淀粉酶基因的转录增加引起的,而是由α-淀粉酶的更快分泌或由α-淀粉酶与生物质的较低结合引起的。 [参考:12]

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