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Comprehensive model of microalgae photosynthesis rate as a function of culture conditions in photobioreactors

机译:光合作用中微藻光合作用速率与培养条件的综合模型

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In this paper, the influence of culture conditions (irradiance, temperature, pH, and dissolved oxygen) on the photosynthesis rate of Scenedesmus almeriensis cultures is analyzed. Short-run experiments were performed to study cell response to variations in culture conditions, which take place in changing environments such as outdoor photobioreactors. Experiments were performed by subjecting diluted samples of cells to different levels of irradiance, temperature, pH, and dissolved oxygen concentration. Results demonstrate the existence of photoinhibition phenomena at irradiances higher than 1,000 μE/m~2 s; in addition to reduced photosynthesis rates at inadequate temperatures or pH-the optimal values being 35 °C and 8, respectively. Moreover, photosynthesis rate reduction at dissolved oxygen concentrations above 20 mg/l is demonstrated. Data have been used to develop an integrated model based on considering the simultaneous influence of irradiance, temperature, pH, and dissolved oxygen. The model fits the experimental results in the range of culture conditions tested, and it was validated using data obtained by the simultaneous variation of two of the modified variables. Furthermore, the model fits experimental results obtained from an outdoor culture of S. almeriensis performed in an open raceway reactor. Results demonstrate that photosynthetic efficiency is modified as a function of culture conditions, and can be used to determine the proximity of culture conditions to optimal values. Optimal conditions found (T=35 °C, pH=8, dissolved oxygen concentration <20mg/l) allows to maximize the use of light by the cells. The developed model is a powerful tool for the optimal design and management of microalgae-based processes, especially outdoors, where the cultures are subject to daily culture condition variations.
机译:本文分析了培养条件(辐照度,温度,pH和溶解氧)对Scendesmus almeriensis培养物光合作用速率的影响。进行了短期实验,以研究细胞对培养条件变化的反应,这种变化发生在不断变化的环境(例如室外光生物反应器)中。通过将稀释后的细胞样品置于不同水平的辐照度,温度,pH和溶解氧浓度下进行实验。结果表明,在高于1000μE/ m〜2 s的辐照下存在光抑制现象。除了在不足的温度或pH值下降低光合作用速率外,最佳值分别为35°C和8。此外,证明了在溶解氧浓度高于20 mg / l时光合作用速率降低。考虑到辐照度,温度,pH和溶解氧的同时影响,已使用数据来开发集成模型。该模型适合于所测试培养条件范围内的实验结果,并使用通过同时改变两个修饰变量获得的数据进行了验证。此外,该模型适合从露天赛道反应堆中进行的阿尔门链球菌室外培养获得的实验结果。结果表明,光合作用效率随培养条件而变化,可用于确定培养条件与最佳值的接近度。发现最佳条件(T = 35°C,pH = 8,溶解氧浓度<20mg / l)可以使细胞最大限度地利用光。开发的模型是用于基于微藻的工艺(尤其是室外)的优化设计和管理的强大工具,在室外,培养物每天的培养条件都会变化。

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