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首页> 外文期刊>Applied Microbiology and Biotechnology >A comparison of two 16S rRNA gene-based PCR primer sets in unraveling anammox bacteria from different environmental samples
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A comparison of two 16S rRNA gene-based PCR primer sets in unraveling anammox bacteria from different environmental samples

机译：比较两种基于16S rRNA基因的PCR引物在不同环境样品中分离的厌氧氨氧化细菌中的比较

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摘要

Two 16S rRNA gene-based PCR primer sets (Brod541F/Amx820R and A438f/A684r) for detecting anammox bacteria were compared using sediments from Mai Po wetlands (MP), the South China Sea (SCS), a freshwater reservoir (R2), and sludge granules from a wastewater treatment plant (A2). By comparing their ability in profiling anammox bacteria, the recovered diversity, community structure, and abundance of anammox bacteria among all these diverse samples indicated that A438f/A684r performed better than Brod541F/Amx820R in retrieving anammox bacteria from these different environmental samples. Five Scalindua subclusters (zhenghei-I, SCS-I, SCS-III, arabica, and brodae) dominated in SCS whereas two Scalindua subclusters (zhenghei-II and wagneri) and one cluster of Kuenenia dominated in MP. R2 showed a higher diversity of anammox bacteria with two new retrieved clusters (R2-New-1 and R2- New-2), which deserves further detailed study. The dominance of Brocadia in sample A2 was supported by both of the primer sets used. Results collectively indicate strongly niche-specific community structures of anammox bacteria in different environments, and A438f/A684r is highly recommended for screening anammox bacteria from various environments when dealing with a collection of samples with diverse physiochemical characteristics.
机译：使用来自米埔湿地（MP），南海（SCS），淡水水库（R2）和海底生物的沉积物，比较了两个基于16S rRNA基因的PCR引物组（Brod541F / Amx820R和A438f / A684r），用于检测厌氧细菌。来自废水处理厂（A2）的污泥颗粒。通过比较它们在厌氧性厌氧菌中进行分析的能力，在所有这些不同样品中厌氧性厌氧菌的恢复多样性，群落结构和丰度表明，A438f / A684r在从这些不同环境样品中回收厌氧性厌氧菌方面比Brod541F / Amx820R表现更好。在SCS中，五个Scalindua子群（Zhenggei-I，SCS-1，SCS-III，阿拉伯咖啡和婆罗洲）占主导地位，而在MP中，两个Scalindua子群（zhenghei-II和wagneri）和一个Kuenenia集群占主导地位。 R2具有两个新检索到的簇（R2-New-1和R2-New-2），显示出较高的厌氧细菌多样性，值得进一步详细研究。所用的两个引物组都证明了Brocadia在样品A2中的优势地位。结果共同表明在不同环境中厌氧菌具有很强的生态位特异性社区结构，强烈建议使用A438f / A684r在处理具有多种理化特性的样品时从各种环境中筛选厌氧菌。

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