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首页> 外文期刊>Applied Microbiology and Biotechnology >Identification and production of a novel natural pigment, cordycepoid A, from Cordyceps bifusispora
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Identification and production of a novel natural pigment, cordycepoid A, from Cordyceps bifusispora

机译：从冬虫夏草中鉴定和生产新型天然色素虫草素A

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摘要

A novel yellow pigment, cordycepoid A, was isolated and identified from the entomogenous fungi Cordyceps bifusispora. Cordycepoid A exhibited no significant toxicity against Chinese hamster ovary (CHO) cells and mice, and showed high stability against food addictives, metal ions and heat. A liquid/solid double-phase cultural process for the production of the pigment was optimized as follows: 3 days aged liquid seed, 7.5 % inoculums, incubation temperature at 25 C, 10 days of solid culture, and the last 5 days exposed to 200 Lx scattered light. The liquid seed medium and the solid culture medium were also optimized. Ethanol was selected as extracting solvent for its scale-up production. The optimal extracting conditions were determined as liquid/solid ratio at 20:1, extracting temperature at 40 C, ultrasonic power at 400 W, and extracting time of 40 min.
机译：从虫源真菌冬虫夏草中分离并鉴定了一种新型的黄色色素虫草素A。虫草素A对中国仓鼠卵巢（CHO）细胞和小鼠没有明显的毒性，对食物成瘾剂，金属离子和热量也显示出很高的稳定性。如下优化了用于生产颜料的液/固双相培养过程：老化3天的液体种子，7.5％的接种量，25°C的孵育温度，10天的固体培养以及最后5天暴露于200 Lx散射光。还优化了液体种子培养基和固体培养基。选择乙醇作为提取溶剂以扩大规模。确定最佳提取条件为液/固比为20：1，提取温度为40℃，超声功率为400 W，提取时间为40分钟。

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