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首页> 外文期刊>Applied Microbiology and Biotechnology >A novel psychrophilic lipase from Pseudomonas fluorescens with unique property in chiral resolution and biodiesel production via transesterification
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A novel psychrophilic lipase from Pseudomonas fluorescens with unique property in chiral resolution and biodiesel production via transesterification

机译：荧光假单胞菌的一种新型嗜冷脂肪酶，在手性拆分和通过酯交换反应生产生物柴油方面具有独特的特性

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摘要

A lipase-producing bacterium strain B68 screened from soil samples of China was identified as Pseudomonas fluorescens. With GenomeWalker, the open reading frame of lipase gene lipB68, encoding 476 amino acids, was cloned and expressed in Escherichia coli BL21 (DE3). By affinity chromatography, the recombinant LipB68 protein was purified to the purity of 95%. As a member of lipase subfamily I.3, LipB68 has a unique optimum temperature of 20 degrees C, which was the lowest in this subfamily. In chiral resolution, LipB68 effectively catalyzed the transesterification of both alpha-phenylethanol and alpha-phenylpropanol at 20 degrees C, achieving E values greater than 100 and 60 after 120 h, respectively. Among all the known catalysts in biodiesel production, LipB68 produced biodiesel with a yield of 92% after 12 h, at the lowest temperature of 20 degrees C, and is the first one of the I.3 lipase subfamily reported to be capable of catalyzing the transesterification reaction of biodiesel production. Since lipasemediated biodiesel production is normally carried out at 35-50 degrees C, the availability of a highly active lipase with a low optimal temperature can provide substantial savings in energy consumption. Thus, this novel psychrophilic lipase (LipB68) may represent a highly competitive energy-saving biocatalyst.
机译：从中国土壤样品中筛选出的产脂肪酶细菌菌株B68被鉴定为荧光假单胞菌。利用GenomeWalker，克隆了编码476个氨基酸的脂肪酶基因lipB68的开放阅读框，并在大肠杆菌BL21（DE3）中表达。通过亲和色谱，将重组LipB68蛋白纯化至95％的纯度。作为脂肪酶亚家族I.3的成员，LipB68具有20摄氏度的独特最佳温度，这是该亚家族中最低的。在手性拆分中，LipB68在20摄氏度下有效催化了α-苯基乙醇和α-苯基丙醇的酯交换反应，在120 h后分别达到了大于100和60的E值。在生物柴油生产中所有已知的催化剂中，LipB68在20℃的最低温度下12小时后生产的生物柴油的收率为92％，并且是I.3脂肪酶亚家族中第一个能够催化生物柴油生产的催化剂。生物柴油生产的酯交换反应。由于脂肪酶介导的生物柴油生产通常在35至50摄氏度下进行，因此可获得具有较低最佳温度的高活性脂肪酶可以大大节省能源消耗。因此，这种新型的嗜冷脂肪酶（LipB68）可以代表一种高度竞争的节能生物催化剂。

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