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Changes in the potential functional diversity of the bacterial community in biofilters

机译：生物滤池中细菌群落潜在功能多样性的变化

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摘要

The bacterial community structure in a biofilter treating ethanol was investigated using community level physiological profiling. Laboratory scale biofilters of two sizes (5 or 11.5 cm internal diameter with 30 or 67 cm packed height, respectively) were packed with compost and a humidified airstream loaded with ethanol passed through them. Good removal efficiencies (82-100%) and elimination capacities (49-205 g ethanol m(-3) h(-1)) were observed in all units. Compost packing media samples were extracted and the community level physiological profiles assayed using Biolog Ecoplates. The community structure was found to be similar over a range of a few centimetres. No differences were observed between sample sizes of 0.5-1 and 6 g, and therefore, the smaller sample size (typical of that used in previous studies) is appropriate for use in the future. Two studies of parallel systems showed that the community structure diverged during the acclimation period (10 days) in one pair, but in another pair, no divergence was observed and a similar shift in community profile was observed in both units between 25 and 40 days of operation. Community level physiological profiling with Biolog Ecoplates is a useful method for detecting differences between and changes within the bacterial communities in biofilters.
机译：使用社区水平的生理概况研究生物滤池中处理乙醇的细菌群落结构。将两种尺寸（内径分别为5或11.5厘米，包装高度分别为30或67厘米）的实验室规模生物过滤器装满堆肥，并在其中加入装有乙醇的加湿气流。在所有单元中均观察到良好的去除效率（82-100％）和消除能力（49-205 g乙醇m（-3）h（-1））。提取堆肥包装介质样品，并使用Biolog Ecoplates测定群落水平的生理特征。发现社区结构在几厘米的范围内相似。在0.5-1和6 g的样本量之间未观察到差异，因此，较小的样本量（通常是先前研究中使用的样本量）适合将来使用。两项平行系统研究表明，在适应期（10天）中，一对动物群落结构发生了差异，但在另一对中，没有观察到差异，并且在25到40天之间，两个单位的群落分布都发生了类似的变化。操作。使用Biolog Ecoplates进行社区水平的生理概况分析是检测生物滤池中细菌群落之间差异和变化的有用方法。

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