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首页> 外文期刊>Applied Microbiology and Biotechnology >Single-stage autotrophic nitrogen-removal process using a composite matrix immobilizing nitrifying and sulfur-denitrifying bacteria
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Single-stage autotrophic nitrogen-removal process using a composite matrix immobilizing nitrifying and sulfur-denitrifying bacteria

机译：使用固定化硝化和脱硫细菌的复合基质的单级自养脱氮工艺

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摘要

We developed a novel single-stage autotrophic nitrogen-removal process comprised of two composite immobilized biomass layers-one of nitrifying bacteria and one of sulfar-denitrifying bacteria and elemental sulfur-in a Fe-Ni fibrous slag matrix. Nitrification and consumption of dissolved oxygen occurred in the outer part and sulfur denitrification in the anoxic inner part of the composite matrix, thus realizing autotrophic nitrogen removal in a single reactor. The complete conversion of ammonia into N-2 in a single reactor was demonstrated in both batch-mode incubation and continuous-feed operation. The spatial profiles of the ammonia-oxidizing bacteria and denitrifying bacteria were evaluated by real-time PCR, targeting their functional genes, and stratification of these two types was observed in the matrix after several months of incubation. This process does not require any specific reactor type or conditions and thus has the potential to be applied to many different wastewater treatment processes due to its simplicity in both operation and construction.
机译：我们开发了一种新颖的单级自养脱氮工艺，该工艺包括在Fe-Ni纤维渣基质中由两个复合固定化生物质层组成-一个硝化细菌层，一个亚硫酸盐脱氮细菌和元素硫层。复合基质的外部发生硝化和溶解氧的消耗，而在复合基质的缺氧内部发生硫的反硝化，从而在单个反应器中实现自养氮的去除。分批模式培养和连续进料操作均证明了氨在单个反应器中完全转化为N-2。通过实时PCR评估氨氧化细菌和反硝化细菌的空间分布，靶向它们的功能基因，并在孵育几个月后在基质中观察到这两种类型的分层。该方法不需要任何特定的反应器类型或条件，并且由于其操作和构造简单，因此有潜力应用于许多不同的废水处理过程。

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