• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Microbiology and Biotechnology >Competition for Astragalus sinicus root nodule infection between its native microsymbiont Rhizobium huakuii bv. renge B3 and Rhizobium sp. ACMP18 strain, specific for Astragalus cicer
【24h】

Competition for Astragalus sinicus root nodule infection between its native microsymbiont Rhizobium huakuii bv. renge B3 and Rhizobium sp. ACMP18 strain, specific for Astragalus cicer

机译：在其天然微共生体华枯根霉之间竞争黄芪根瘤。 renge B3和根瘤菌sp。 ACMP18菌株，特异于黄芪

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Rhizobium huakuii bv. renge B3, a native symbiont of Astragalus sinicus, outcompeted Rhizobium sp. strain ACMP18, which was isolated from Astragalus cicer nodules, in the formation of root nodules on A. sinicus when plants were co-inoculated with these strains. The strains occupying the nodules were identified by antibiotic resistance and phage sensitivity markers and also by polymerase chain reaction (PCR) genomic fingerprinting, using enterobacterial repetitive intergenic consensus sequences. InPCR genomic fingerprinting, the total genomic DNA isolated from pure bacterial culture and from squashed root nodules showed identical profiles, indicating that this technique can be a useful tool for identification of rhizobia in ecological studies. When Rhizobium sp. strain ACMP18 outnumbered R. huakuii bv. renge strain B3 by a factor of ten, and even when strain ACMP18 was added to plants 1 week before bacterization with strain B3, the strain B3 occupied most nodules. Dually infected nodules were notobserved, although Rhizobium sp. ACMP18 formed active nodules on A. sinicus when the bacterial strain was inoculated alone.
机译：华夏根瘤菌renge B3是黄芪的天然共生菌，胜过根瘤菌。当植物与这些菌株共同接种时，从黄芪的结节中分离出的ACMP18菌株在中华曲霉上形成根瘤。使用肠杆菌重复基因间共有序列，通过抗生素抗性和噬菌体敏感性标记以及聚合酶链反应（PCR）基因组指纹图谱鉴定占据根瘤的菌株。在PCR基因组指纹图谱中，从纯细菌培养物和挤压的根瘤中分离出的总基因组DNA表现出相同的特征，这表明该技术可以作为在生态学研究中鉴定根瘤菌的有用工具。当根瘤菌sp。菌株ACMP18的数量超过华氏双歧杆菌。将B3菌株还原10倍，甚至在菌株B3杀菌前1周将ACMP18菌株添加到植物中时，B3菌株仍占据大部分结节。没有观察到双重感染的结核，尽管根瘤菌属。当单独接种细菌菌株时，ACMP18在中华曲霉上形成了活动结节。

著录项

相似文献

  • 外文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号