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首页> 外文期刊>Applied Microbiology and Biotechnology >Production of cellulase by a wild strain of Chaetomium globosum using delignified oil palm empty-fruit-bunch fibre as substrate
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Production of cellulase by a wild strain of Chaetomium globosum using delignified oil palm empty-fruit-bunch fibre as substrate

机译：以脱木质的油棕空果束纤维为底物，由球形拟南芥野生菌株生产纤维素酶

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摘要

Studies on the feasibility of using delignified oil palm empty-fruit-bunch (OPEFB) fibres as a substrate for cellulase production by Chaetomium globosum strain 414 were carried out in shake-flask cultures containing different types and concentrations of nitrogen source. Peptone, as nitrogen source, gave maximum production of all the three main components of the cellulase complex (endoglucanase or carboxymethylcellulase, cellobiohydrolase or filter-paper-hydrolysing enzyme and beta-glucosidase), followed by yeast extract, urea, KNO3 and (NH4)(2)SO4. The maximum specific growth rate (mu(max)) of C. globosum strain 414 grown in medium containing OPEFB and peptone was 0.038 h(-1). In all the fermentations, the fungus was able to produce all the three cellulases with significant amounts of beta-glucosidase, except when using (NH4)(2)SO4 as nitrogen source, where beta-glucosidase was not produced. With 6 g/l peptone and 10 g/l delignified OPEFB fibres, the fungus produced maximum concentrations of FPase, carboxymethylcellulase and beta-glucosidase: 1.4, 30.8 and 9.8 U/ml, giving productivities of 10, 214 and 24 U l(-1) h(-1), respectively. The cellulase mixture, partially purified by ammonium sulphate precipitation, was able to hydrolyse delignified OPEFB fibres, converting about 68% of the cellulosics to reducing sugars after 5 days.
机译：在含有不同类型和浓度氮源的摇瓶培养物中，进行了使用脱木质素的油棕空果束（OPEFB）纤维作为生产球壳拟南杆菌菌株414的纤维素酶的可行性的研究。蛋白nitrogen作为氮源，可使纤维素酶复合物的所有三个主要成分（内切葡聚糖酶或羧甲基纤维素酶，纤维二糖水解酶或滤纸水解酶和β-葡萄糖苷酶）的产量最大化，其次是酵母提取物，尿素，KNO3和（NH4） （2）SO4。在包含OPEFB和蛋白ept的培养基中生长的球梭菌414的最大比生长速率（mu（max））为0.038 h（-1）。在所有的发酵过程中，真菌都能够生产出三种纤维素酶，并带有大量的β-葡萄糖苷酶，但使用（NH4）（2）SO4作为氮源时，则不会产生β-葡萄糖苷酶。使用6 g / l蛋白ept和10 g / l去木质素的OPEFB纤维，该真菌产生的FPase，羧甲基纤维素酶和β-葡萄糖苷酶的最大浓度分别为1.4、30.8和9.8 U / ml，生产率分别为10、214和24 U l（- 1）h（-1）。通过硫酸铵沉淀部分纯化的纤维素酶混合物能够水解脱木质素的OPEFB纤维，在5天后将约68％的纤维素转化为还原糖。

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