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Nano-mechanical single-cell sensing of cell-matrix contacts

机译：cell-matrix Nano-mechanical单细胞传感联系人

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Extracellular protein matrices provide a rigidity interface exhibiting nano-mechanical cues that guide cell growth and proliferation. Cells sense such cues using actin-rich filopodia extensions which encourage favourable cell-matrix contacts to recruit more actin-mediated local forces into forming stable focal adhesions. A challenge remains in identifying and measuring these local cellular forces and in establishing empirical relationships between them, cell adhesion and filopodia formation. Here we investigate such relationships using a micromanipulation system designed to operate at the time scale of focal contact dynamics, with the sample frequency of a force probe being 0.1 ms, and to apply and measure forces at nano-to-micro Newton ranges for individual mammalian cells. We explore correlations between cell biomechanics, cell-matrix attachment forces and the spread areas of adhered cells as well as their relative dependence on filopodia formation using synthetic protein matrices with a proven ability to induce enhanced filopodia numbers in adherent cells. This study offers a basis for engineering exploitable cell-matrix contacts in situ at the nanoscale and single-cell levels.
机译：细胞外蛋白矩阵提供刚性界面展示nano-mechanical暗示引导细胞生长和增殖。这样的提示使用actin-rich丝状伪足扩展这鼓励有利cell-matrix联系人招募更多actin-mediated当地部队形成稳定的焦粘连。仍在确定和测量这些地方细胞力量和建立经验它们之间的关系,细胞粘附和丝状伪足的形成。使用精密控制系统的关系设计操作时间尺度的焦点接触动力学的采样频率力探针是0.1毫秒,和应用测量nano-to-micro牛顿力范围个人的哺乳动物细胞。细胞生物力学之间的相关性,cell-matrix附件部队和传播领域坚持细胞以及它们的相对使用合成依赖丝状伪足的形成诱导蛋白矩阵与证明能力增强的丝状伪足数字贴壁细胞。这项研究提供了一个基础工程利用cell-matrix联系人在原地纳米尺度和单细胞水平。

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