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首页> 外文期刊>EMBO Journal >UBPY: a growth-regulated human ubiquitin isopeptidase.
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UBPY: a growth-regulated human ubiquitin isopeptidase.

机译：UBPY: growth-regulated人类泛素isopeptidase。

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The ubiquitin pathway has been implicated in the regulation of the abundance of proteins that control cell growth and proliferation. We have identified and characterized a novel human ubiquitin isopeptidase, UBPY, which both as a recombinant protein and upon immunoprecipitation from cell extracts is able to cleave linear or isopeptide-linked ubiquitin chains. UBPY accumulates upon growth stimulation of starved human fibroblasts, and its levels decrease in response to growth arrest induced by cell-cell contact. Inhibition of UBPY accumulation by antisense plasmid microinjection prevents fibroblasts from entering S-phase in response to serum stimulation. By increasing or decreasing the cellular abundance of UBPY or by overexpressing a catalytic site mutant, we detect substantial changes in the total pattern of protein ubiquitination, which correlate stringently with cell proliferation. Our results suggest that UBPY plays a role in regulating the overall function of the ubiquitin-proteasome pathway. Affecting the function of a specific UBP in vivo could provide novel tools for controlling mammalian cell proliferation.
机译：泛素通路已经被涉及调节蛋白质的丰度控制细胞生长和增殖。特征识别和小说的人泛素isopeptidase UBPY,既是重组蛋白和免疫沉淀反应从细胞中提取能够线性或裂开isopeptide-linked泛素链。积累对经济增长的刺激饿死了人类成纤维细胞,其水平下降应对增长信息引起的逮捕接触。反义质粒显微注射预防成纤维细胞进入s阶段来响应血清刺激。UBPY或细胞丰度overexpressing催化部位变异,我们检测到大量的总模式的变化蛋白质泛素化相关严格的细胞增殖。表明UBPY在调节过程中发挥作用ubiquitin-proteasome的整体功能途径。体内可以提供新颖的控制工具哺乳动物的细胞增殖。

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