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首页> 外文期刊>Applied Microbiology and Biotechnology >Identification and characterization of a mosquito pupicidal metabolite of a Bacillus subtilis subsp. subtilis strain
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Identification and characterization of a mosquito pupicidal metabolite of a Bacillus subtilis subsp. subtilis strain

机译：枯草芽孢杆菌亚种的蚊杀性代谢产物的鉴定和表征。枯草杆菌菌株

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摘要

The culture supernatant of a strain of Bacillus subtilis subsp. subtilis isolated from mangrove forests of Andaman and Nicobar islands, India was found to kill larval and pupal stages of mosquitoes. A chloroform extract of the culture supernatant of the bacterium showed pupicidal effects at an LC _(50) dose of 1 μg/ml. The mosquitocidal metabolite(s) produced by this strain were purified by gel permeation chromatography. The purified fraction was subjected to Fourier transform infrared (FTIR) spectroscopy and Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry. The FTIR spectrum of active fraction/CHCl3 residue showed strong band characteristic of peptides. MALDI-TOF spectrum of the sample showed well-resolved group of peaks at m/z values 1,030.6, 1,046.7, 1,044.6, 1,060.5, 1,058.6, 1,058.7, and 1,074.6. The results indicated production of different isoforms of surfactin, ranging from C13-C15. Further, the sfp gene responsible for the production of surfactin was amplified and sequenced. In conclusion, this study showed that the mosquito pupicidal metabolite(s), produced by B. subtilis subsp. subtilis is the cyclic lipopeptide, surfactin. The mode of action of surfactin on pupae of mosquitoes is discussed. This is the first report on the mosquito pupicidal activity of surfactin produced by B. subtilis subsp. subtilis.
机译：枯草芽孢杆菌亚种菌株的培养上清液。从印度安达曼和尼科巴岛的红树林中分离出的枯草被发现杀死了幼虫和up阶段的蚊子。细菌培养物上清液的氯仿提取物在LC_（50）剂量为1μg/ ml时显示出杀螨作用。由该菌株产生的灭蚊子代谢产物通过凝胶渗透色谱法纯化。对纯化的级分进行傅立叶变换红外（FTIR）光谱和基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱。活性级分/ CHCl3残基的FTIR光谱显示出强的肽带特征。样品的MALDI-TOF谱显示m / z值为1,030.6、1,046.7、1,044.6、1,060.5、1,058.6、1,058.7和1,074.6的一组峰的分辨良好。结果表明产生了表面活性蛋白的不同同工型，范围从C13-C15。此外，负责表面活性素产生的sfp基因被扩增和测序。总之，这项研究表明，枯草芽孢杆菌亚种产生的蚊子杀虫剂代谢产物。枯草杆菌是环状脂肽，表面活性素。讨论了表面活性素对蚊虫p的作用方式。这是关于枯草芽孢杆菌亚种产生的表面活性素的灭蚊活性的首次报道。枯草杆菌。

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