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首页> 外文期刊>EMBO Journal >THE GYPSY INSULATOR CAN FUNCTION AS A PROMOTER-SPECIFIC SILENCER IN THE DROSOPHILA EMBRYO
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THE GYPSY INSULATOR CAN FUNCTION AS A PROMOTER-SPECIFIC SILENCER IN THE DROSOPHILA EMBRYO

机译：吉普赛绝缘子可以作为在果蝇PROMOTER-SPECIFIC消音器胚胎

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The Drosophila gypsy retrotransposon disrupts gene activity by blocking the interactions of distal enhancers with target promoters, This enhancer-blocking activity is mediated by a 340 bp insulator DNA within gypsy. The insulator contains a cluster of binding sites for a zinc finger protein, suppressor of Hairy wing [su(Hw)], Recent studies have shown that a second protein, mod(mdg4), is also important for normal insulator function. Mutations in mod(mdg4) exert paradoxical effects on different gypsy-induced phenotypes, For example, it enhances yellow(2) but suppresses cut(6). Here, we employ a stripe expression assay in transgenic embryos to investigate the role of mod(mdg4) in gypsy insulator activity. The insulator was inserted between defined enhancers and placed among divergently transcribed reporter genes (white and lacZ) containing distinct core promoter sequences. These assays indicate that mod(mdg4) is essential for the enhancer-blocking activity of the insulator DNA, Moreover, reductions in mod(mdg4)(+) activity cause the insulator to function as a promoter-specific silencer that selectively represses white, but not lacZ, The repression of white does not affect the expression of the closely linked lacZ gene, suggesting that the insulator does not propagate changes in chromatin structure, These results provide are explanation for why mod(mdg4) exerts differential effects on different gypsy-induced mutations. [References: 51]
机译：果蝇吉普赛逆转录转座子扰乱基因活动通过阻断远的交互增强子与启动子目标,这一点enhancer-blocking活动是由一个340英国石油(bp)绝缘子DNA在吉普赛。包含一个集群结合位点的锌手指蛋白质,抑制的毛茸茸的翅膀苏(Hw),最近的研究表明,第二个蛋白质,国防部(mdg4),正常也很重要绝缘子功能。对不同gypsy-induced矛盾的影响表型,例如,它增强了黄色(2)但抑制减少(6)。在转基因胚胎表达测定调查在吉普赛mod (mdg4)的作用绝缘子的活动。定义的增强子和放置在之间发散思维转录基因(白色和记者lacZ)包含不同的核心启动子序列。enhancer-blocking活动是至关重要的绝缘子的DNA,此外,减少国防部(mdg4)(+)活动导致绝缘子作为promoter-specific消音器选择性地压制白,但不是lacZ,镇压白不影响lacZ密切相关基因的表达,这表明绝缘子不传播染色质结构的改变,这些结果提供的是解释为什么国防部(mdg4)产生对不同gypsy-induced微分的影响突变。

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