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首页> 外文期刊>Current Protocols in Nucleic Acid Chemistry >Synthesis of 4-Cyanoindole Nucleosides, 4-Cyanoindole-2'-Deoxyribonucleoside-5'-Triphosphate (4CIN-TP), and Enzymatic Incorporation of 4CIN-TP into DNA
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Synthesis of 4-Cyanoindole Nucleosides, 4-Cyanoindole-2'-Deoxyribonucleoside-5'-Triphosphate (4CIN-TP), and Enzymatic Incorporation of 4CIN-TP into DNA

机译：4-Cyanoind 4-Cyanoindole核苷的合成

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摘要

4-Cyanoindole-2'-deoxyribonucleoside (4CIN) is a fluorescent isomorphic nucleoside analogue with superior spectroscopic properties in terms of Stokes shift and quantum yield in comparison to the widely utilized isomorphic nucleoside analogue, 2-aminopurine-2'-deoxyribonucleoside (2APN). Notably, when inserted into single- or double-stranded DNA, 4CIN experiences substantially less in-strand fluorescence quenching compared to 2APN. Given the utility of these properties for a spectrum of research applications involving oligonucleotides and oligonucleotide-protein interactions (e.g., enzymatic processes, DNA hybridization, DNA damage), we envision that additional reagents based on 4-cyanoindole nucleosides may be widely utilized. This protocol expandson the previously published synthesis of 4CIN to include synthetic routes to both 4-cyanoindole-ribonucleoside (4CINr) and 4-cyanoindole-2'-deoxyribonucleoside-5'-triphosphate (4CIN-TP), as well as a method for the enzymatic incorporation of 4CIN-TP intoDNA by a polymerase. These methods are anticipated to further enable the utilization of 4CIN in diverse applications involving DNA and RNA oligonucleotides.
机译：4-Cyanoindole-2的脱氧核苷(4 cin)是一个荧光同构核苷类似物优越的光谱特性斯托克斯位移和量子收益率相比广泛利用同构的核苷模拟,2-aminopurine-2脱氧核苷(2前置。双链DNA, 4 cin的经历大大减少in-strand荧光淬火相比,2前置。这些属性的光谱研究应用寡核苷酸和oligonucleotide-protein交互(例如,酶促过程、DNA杂交的DNA损害),我们设想,额外的试剂基于4-cyanoindole核苷可能是广泛的利用。发表4 cin的合成包括合成路线都4-cyanoindole-ribonucleoside(4 cinr)

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