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首页> 外文期刊>Catalysis science & technology >What makes a lipase a valuable acyltransferase in water abundant medium?
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What makes a lipase a valuable acyltransferase in water abundant medium?

机译：什么使脂肪酶的酰基转移酶水丰富的媒介?

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摘要

The necessity to develop more eco-friendly processes in oleochemistry has recently led to a renewed consideration of lipases exhibiting high acyltransferase activity in hydrated media. Yet, what precisely differentiates a "usual" lipase from a lipase/acyltransferase and why and when the latter can be truly interesting for biocatalysis processes still need to be clearly stated. Here we propose an extensive kinetic characterization of 13 lipases including the well-known lipases A and B from Moesziomyces antarcticus (Candida antarctica) and lipases/acyltransferases homologous to CpLIP2 from Candida parapsilosis. The enzymes were tested in a model reaction medium comprising a fatty acid alkyl ester substrate, an alcohol as a nucleophile in a wide range of concentrations (0.01-12 M) and water at very high thermodynamic activity (a(W) > 0.9). These reaction conditions allowed a clear characterization of the behavior of the different biocatalysts. Principally, lipases/acyltransferases catalyze acyl transfer at a much higher rate than hydrolysis, with fast accumulation of the fatty acid alkyl ester product up to a transient maximum concentration higher than that of the thermodynamic equilibrium, through a kinetically controlled reaction. Experimental determination of particular parameters and kinetic constants led to a classification of the lipases according to their high, average or low acyltransferase character. This study thus proposes a clear definition and a simple methodology to assess the potential of lipases for efficient fatty acid ester synthesis in aqueous media.
机译：开发更环保的必要性过程oleochemistry最近导致了重新考虑脂酶表现出高在媒体水化酰基转移酶的活动。什么精确区分“通常”脂肪酶从脂肪酶/酰基转移酶,为什么当后者可以真正有趣的生物催化过程仍需清楚说明。表征13脂酶包括从Moesziomyces知名脂酶A和Bantarcticus strain(南极假丝酵母)和脂酶/ CpLIP2酰基转移酶同源从假丝酵母parapsilosis。在模型组成的反应介质进行测试脂肪酸烷基酯基质,酒精亲核试剂在范围广泛的浓度(0.01 -12)和水在非常高的热力学活动((W) > 0.9)。允许一个清晰描述的行为不同的生物催化剂。脂酶/酰基转移酶催化酰基转移比水解以更高的速度,太快了脂肪酸烷基酯的积累产品瞬态最大浓度高于热力学平衡,通过一个活动的控制的反应。特定的参数和动力学常数脂酶的分类根据高,平均水平或低酰基转移酶的性格。定义和一个简单的方法来评估脂酶的潜在有效的脂肪酸酯合成水媒体。

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