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首页> 外文期刊>Journal of Cellular Physiology >Vimentin as a Marker of Early Differentiating, Highly Motile Corneal Epithelial Cells
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Vimentin as a Marker of Early Differentiating, Highly Motile Corneal Epithelial Cells

机译：波形蛋白的标记区分早期,角膜上皮细胞高度等

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Vimentin (Vim), a cytoskeletal intermediate filament, is part of a naturally occurring reversible program, the Epithelial-Mesenchymal Transition (EMT), which converts epithelial cells into mesenchymal-like derivatives. Based on previous results showing that epithelial cells co-express Vim and keratin (Krt) as part of a cytoskeletal network which confers them a highly motile phenotype, we explored the role of Vim in rabbit corneal epithelial cells or RCE1(5T5) cells, an established model of corneal epithelial differentiation. Vim and keratin filaments were co-expressed in cells localized at the proliferative/migratory rim of the growing colonies, but not in basal cells from the center of the colonies nor at suprabasal cell layers. Flow cytometry and qPCR demonstrated that there was a decrease in Krt(+)/Vim(+) cell number and Np63 expression when cells reached confluence and formed a 4-5 layered epithelium, while there was a concomitant increase of both Pax-6 expression and Krt(+)/Vim(-) cells. Inhibition of cell proliferation with mitomycin C did not modify cell motility nor the expression of Vim. We studied the distribution and expression of 6 integrin, a protein also involved in cell migration. The results demonstrated that 6 integrin had a distribution which was, in part, co-linear with Vim at the proliferative/migratory rim of cell colonies, suggesting an indirect interaction between these proteins. Immunoprecipitation and immunostaining assays indicated that plectin might be mediating such interaction. These data suggest that Vim expression in corneal epithelium is found in a cell population composed of highly motile cells with a Vim(+)/Krt(+)/Np63(+)/Pax-6(low)/6 integrin(+) phenotype. J. Cell. Physiol. 232: 818-830, 2017. (c) 2016 Wiley Periodicals, Inc.
机译：波形蛋白(Vim),细胞骨架中间丝,是一种天然的一部分Epithelial-Mesenchymal可逆的程序过渡(EMT)将上皮细胞mesenchymal-like衍生品。以前的结果表明上皮细胞co-express Vim和角蛋白(Krt)的一部分细胞骨架网络,赋予他们一个高度能动的表型,我们探索了Vim的作用兔角膜上皮细胞或RCE1 (5 t5)角膜的上皮细胞,建立模型分化。发生在细胞局部增殖/迁徙rim的增长的殖民地,但不是在基底细胞中心的殖民地和suprabasal细胞层。流式细胞术和qPCR证明是一个减少Krt (+) / Vim(+)细胞数量和当细胞达到融合,Np63表达式形成了一个4 - 5层上皮,而有伴随增加的Pax-6表达式和Krt (+) / Vim(-)细胞。扩散与丝裂霉素C没有修改细胞活性和Vim的表达。研究了分布和表达6整合素,蛋白质也参与细胞迁移。整合素分布的,在某种程度上,同线的Vim在增殖/迁徙边缘细胞的殖民地,表明一种间接这些蛋白质之间的相互作用。免疫沉淀反应和免疫染色分析表明plectin可能调解等交互。表达在角膜上皮中发现的高度能动的细胞组成的细胞群Vim (+) / Krt (+) / Np63 (+) / Pax-6(低)/ 6整合蛋白(+)表型。818 - 830年,2017年。

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