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首页> 外文期刊>Journal of Cellular Physiology >Identifying Nuclear Matrix-Attached DNA Across the Genome
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Identifying Nuclear Matrix-Attached DNA Across the Genome

机译：确定核Matrix-Attached DNA穿过基因组

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Experimental approaches to define the relationship between gene expression and nuclear matrix attachment regions (MARs) have given contrasting and method-specific results. We have developed a next generation sequencing strategy to identify MARs across the human genome (MAR-Seq). The method is based on crosslinking chromatin to its nuclear matrix attachment sites to minimize changes during biochemical processing. We used this method to compare nuclear matrix organization in MCF-10A mammary epithelial-like cells and MDA-MB-231 breast cancer cells and evaluated the results in the context of global gene expression ( array analysis) and positional enrichment of gene-regulatory histone modifications (ChIP-Seq). In the normal-like cells, nuclear matrix-attached DNA was enriched in expressed genes, while in the breast cancer cells, it was enriched in non-expressed genes. In both cell lines, the chromatin modifications that mark transcriptional activation or repression were appropriately associated with gene expression. Using this new MAR-Seq approach, we provide the first genome-wide characterization of nuclear matrix attachment in mammalian cells and reveal that the nuclear matrix-associated genome is highly cell-context dependent. (C) 2016 Wiley Periodicals, Inc.
机译：实验方法定义的关系基因表达和核矩阵之间的关系附件区域(火星)对比和method-specific结果。下一代测序策略来识别火星在人类基因组(MAR-Seq)。方法是基于交联的染色质核基质附着网站最小化在生化处理过程中变化。这种方法比较核矩阵组织MCF-10A乳腺epithelial-like细胞和乳腺癌mda - mb - 231细胞评估结果在全球的背景下基因表达(阵列分析)和位置浓缩的基因调控组蛋白修改(ChIP-Seq)。细胞,核matrix-attached DNA纯度在表达基因,而在乳腺癌细胞,这是富含non-expressed基因。这两种细胞系,染色质的修改马克转录激活或镇压适当地与基因有关吗表达式。提供的第一个全基因组特征在哺乳动物细胞和核矩阵依恋显示,核矩阵的基因组高度依赖cell-context。期刊、公司。

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