• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Cellular Physiology >Appl1 and Appl2 are Expendable for Mouse Development But Are Essential for HGF-Induced Akt Activation and Migration in Mouse Embryonic Fibroblasts
【24h】

Appl1 and Appl2 are Expendable for Mouse Development But Are Essential for HGF-Induced Akt Activation and Migration in Mouse Embryonic Fibroblasts

机译：Appl1和Appl2是鼠标的消耗品发展但HGF-Induced是必不可少的一种蛋白激酶活化和迁移在老鼠胚胎成纤维细胞

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Although Appl1 and Appl2 have been implicated in multiple cellular activities, we and others have found that Appl1 is dispensable for mouse embryonic development, suggesting that Appl2 can substitute for Appl1 during development. To address this possibility, we generated conditionally targeted Appl2 mice. We found that ubiquitous Appl2 knockout (Appl2-/-) mice, much like Appl1-/- mice, are viable and grow normally to adulthood. Intriguingly, when Appl1-/- mice were crossed with Appl2-/- mice, we found that homozygous Appl1;Appl2 double knockout (DKO) animals are also viable and grossly normal with regard to reproductive potential and postnatal growth. Appl2-null and DKO mice were found to exhibit altered red blood cell physiology, with erythrocytes from these mice generally being larger and having a more irregular shape than erythrocytes from wild type mice. Although Appl1/2 proteins have been previously shown to have a very strong interaction with phosphatidylinositol-3 kinase (Pi3k) in thymic T cells, Pi3k-Akt signaling and cellular differentiation was unaltered in thymocytes from Appl1;Appl2 (DKO) mice. However, Appl1/2-null mouse embryonic fibroblasts exhibited defects in HGF-induced Akt activation, migration, and invasion. Taken together, these data suggest that Appl1 and Appl2 are required for robust HGF cell signaling but are dispensable for embryonic development and reproduction. J. Cell. Physiol. 231: 1142-1150, 2016. (c) 2015 Wiley Periodicals, Inc.
机译：尽管Appl1和Appl2已经涉及多个细胞活动,我们和其他人老鼠发现Appl1是可有可无的胚胎发育,表明Appl2即可在开发过程中代替Appl1。解决这种可能性,我们生成的有条件地目标Appl2老鼠。无处不在的Appl2淘汰赛(Appl2 - / -)小鼠,太多像Appl1 - / -小鼠,正常是可行的和成长到成年。越过了Appl2 - / -小鼠,我们发现纯合子Appl1; Appl2双淘汰赛(DKO)动物也是可行的,非常正常关于生殖潜力和产后增长。展览红细胞生理改变,从这些小鼠红细胞一般更大、更不规则的形状从野生型小鼠红细胞。Appl1/2蛋白质已被证明有很强的互动吗phosphatidylinositol-3激酶(Pi3k)胸腺T细胞,Pi3k-Akt信号和细胞在胸腺细胞分化是不变的Appl1; Appl2 (DKO)老鼠。小鼠胚胎成纤维细胞表现出缺陷HGF-induced Akt激活、迁移和入侵。Appl1和Appl2需要健壮的胶质瘤细胞信号但对胚胎是可有可无的发展和繁殖。231: 1142 - 1150, 2016。公司。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号