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首页> 外文期刊>Journal of Cellular Physiology >Proteomics applied to exercise physiology: A cutting-edge technology
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Proteomics applied to exercise physiology: A cutting-edge technology

机译：蛋白质组学应用于运动生理学:尖端技术

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摘要

Exercise research has always drawn the attention of the scientific community because it can be widely applied to sport training, health improvement, and disease prevention. For many years numerous tools have been used to investigate the several physiological adaptations induced by exercise stimuli. Nowadays a closer look at the molecular mechanisms underlying metabolic pathways and muscular and cardiovascular adaptation to exercise are among the new trends in exercise physiology research. Considering this, to further understand these adaptations as well as pathology attenuation by exercise, several studies have been conducted using molecular investigations, and this trend looks set to continue. Through enormous biotechnological advances, proteomic tools have facilitated protein analysis within complex biological samples such as plasma and tissue, commonly used in exercise research. Until now, classic proteomic tools such as one- and two-dimensional polyacrylamide gel electrophoresis have been used as standard approaches to investigate proteome modulation by exercise. Furthermore, other recently developed in gel tools such as differential gel electrophoresis (DIGE) and gel-free techniques such as the protein labeling methods (ICAT, SILAC, and iTRAQ) have empowered proteomic quantitative analysis, which may successfully benefit exercise proteomic research. However, despite the three decades of 2-DE development, neither classic nor novel proteomic tools have been convincingly explored by exercise researchers. To this end, this review gives an overview of the directions in which exercise-proteome research is moving and examines the main tools that can be used as a novel strategy in exercise physiology investigation.
机译：运动的研究一直备受关注科学界的,因为它可以广泛应用于运动训练、健康改善和预防疾病。年众多工具被用来探讨几种生理适应性运动引起的刺激。看看背后的分子机制代谢途径和肌肉心血管适应运动等运动生理学研究的新趋势。考虑到这一点,进一步理解这些适应性以及病理学衰减锻炼,进行了研究使用分子调查,这一趋势似乎仍将继续。生物技术的发展,蛋白质组学工具促进蛋白质分析在复杂生物样品如血浆和组织,常用的运动研究。比如,经典的蛋白质组学工具二维聚丙烯酰胺凝胶电泳作为标准方法研究蛋白质组调制锻炼。在凝胶工具,如微分凝胶电泳(消化)和gel-free技术如蛋白质标记方法(ICAT、SILAC和iTRAQ)授权的蛋白质组学定量分析,可能成功锻炼的蛋白质组学研究中获益。尽管两种发展的三十年,无论是经典还是小说蛋白质组学工具被锻炼令人信服地探索研究人员。概述的方向exercise-proteome研究正在和检查最主要的工具,可以用来作为小说在运动生理学研究的战略。

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