Possible role of RKIP in cytotrophoblast migration: Immunohistochemical and in vitro studies

CiarmelaP.; MarzioniD.; IslamM.S.GrayP.C.TerraccianoL.LorenziT.TodrosT.PetragliaF.CastellucciM.

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Possible role of RKIP in cytotrophoblast migration: Immunohistochemical and in vitro studies

机译：RKIP在细胞滋养层的作用迁移:免疫组织化学和体外研究

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Raf kinase inhibitor protein (RKIP) regulates growth and differentiation signaling of mitogen-activated protein kinases (MAPK), GRK2 and NF-kappaB pathways each of which regulates cytotrophoblast differentiation and normal placental development. We show here that RKIP is expressed in human normal and preeclampic placentas as detected by immunostaining. RKIP was detected in villous cytotrophoblast in normal placenta and switched to syncytiotrophoblast in pre-eclampsia (PE)-complicated pregnancies. RKIP was also localized in extravillous cytotrophoblast of cell islands and cell columns both in normal and in PE placentas, although staining was less uniform in the latter specimens. In order to test RKIP involvement in cytotrophoblast function, we performed in vitro studies on HTR-8/SVneo cells, a first trimester cytotrophoblast cell line. We show that the RKIP inhibitor locostatin reduces ERK phosphorylation and impairs HTR-8/SV neo cells motility in wound closure experiments. We also document the presence of GRK2 mRNA, the reduction of phosphorylated RKIP expression by locostatin and the induction of PAI mRNA expression in HTR-8/SV neo cells, suggesting the involvement of GRK2 and NF-kappaB pathways in these cells. In conclusion, our work provides evidence that RKIP is a novel factor expressed in cytotrophoblast cells where it likely regulates cell migration.

机译：英国皇家空军蛋白质激酶抑制剂(RKIP)调节生长和分化的信号GRK2增殖蛋白激酶(MAPK)和NF-kappaB通路的调节细胞滋养层细胞分化和正常胎盘的发展。在人类正常和preeclampic表示胎盘被免疫染色。发现在正常绒毛细胞滋养层胎盘和转向合胞体滋养层先兆子痫(PE)复杂怀孕。也在extravillous本地化吗细胞滋养层细胞的岛屿和细胞列在正常和PE胎盘,虽然染色不均匀在后者标本。细胞滋养层函数,我们进行了体外研究HTR-8 / SVneo细胞,前三个月细胞滋养层细胞。抑制剂locostatin减少ERK的磷酸化和损害HTR-8 / SV neo细胞运动性伤口关闭实验。GRK2 mRNA,减少磷酸化RKIP locostatin和表达式PAI mRNA表达的诱导HTR-8 / SV新细胞,这表明GRK2的参与在这些细胞NF-kappaB通路。我们的工作提供了证据表明RKIP一部小说因子表达在细胞滋养层细胞它可能调节细胞迁移。

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来源
《Journal of Cellular Physiology》 |2012年第5期|1821-1828|共8页
作者
CiarmelaP.; MarzioniD.; IslamM.S.GrayP.C.TerraccianoL.LorenziT.TodrosT.PetragliaF.CastellucciM.;
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作者单位

Obstetrics and Gynecology, Department of Pediatrics, Obstetrics and Reproductive Medicine;

Faculty of Medicine, Department of Experimental and Clinical Medicine, Polytechnic University of;

Clayton Foundation Laboratories for Peptide Biology, The Salk Institute for Biological Studies, LaDepartment of Obstetrics and Gynecology, University of Turin, Turin, ItalyInstitute for Pathology, University Hospital Basel, Basel, Switzerland;

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原文格式 PDF
正文语种英语
中图分类细胞生物学;
关键词
In vitro; Alternative NF-kappaB Signaling Pathway; Cytotrophoblastsplacenta normalAtypical NF-kappaB Signaling PathwayCanonical NF-kappaB Signaling PathwayG protein coupled receptor kinase 2;

机译：体外;替代NF-kappaB信号途径;Cytotrophoblastsplacenta normalAtypicalNF-kappaB信号PathwayCanonical NF-kappaB信号PathwayG受体蛋白质耦合激酶2;

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Possible role of RKIP in cytotrophoblast migration: Immunohistochemical and in vitro studies

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