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首页> 外文期刊>Journal of Cellular Physiology >Live cell imaging of the cancer-related transcription factor RUNX2 during mitotic progression.
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Live cell imaging of the cancer-related transcription factor RUNX2 during mitotic progression.

机译：癌症相关的活细胞成像转录因子在有丝分裂期间RUNX2进展。

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摘要

The nuclear matrix bound transcription factor RUNX2 is a lineage-specific developmental regulator that is linked to cancer. We have previously shown that RUNX2 controls transcription of both RNA polymerase II genes and RNA polymerase I-dependent ribosomal RNA genes. RUNX2 is epigenetically retained through mitosis on both classes of target genes in condensed chromosomes. We have used fluorescence recovery after photobleaching to measure the relative binding kinetics of enhanced green fluorescent protein (EGFP)-RUNX2 at transcription sites in the nucleus and nucleoli during interphase, as well as on mitotic chromosomes. RUNX2 becomes more strongly bound as cells go from interphase through prophase, with a doubling of the most tightly bound "immobile fraction." RUNX2 exchange then becomes much more facile during metaphase to telophase. During interphase the less tightly bound pool of RUNX2 exchanges more slowly at nucleoli than at subnuclear foci, and the non-exchanging immobile fraction is greater in nucleoli. These results are consistent with a model in which the molecular mechanism of RUNX2 binding is different at protein-coding and ribosomal RNA genes. The binding interactions of RUNX2 change as cells go through mitosis, with binding affinity increasing as chromosomes condense and then decreasing through subsequent mitotic phases. The increased binding affinity of RUNX2 at mitotic chromosomes may reflect its epigenetic function in "bookmarking" of target genes in cancer cells.
机译：核转录因子矩阵RUNX2 lineage-specific发育监管机构与癌症。先前表明RUNX2控制转录的RNA聚合酶II基因和RNA聚合酶I-dependent核糖体RNA基因。通过有丝分裂RUNX2 epigenetically保留在这两类压缩的目标基因染色体。光漂白后测量的相对绑定增强型绿色荧光动力学在转录蛋白(EGFP) -RUNX2网站在间期细胞核和核仁,有丝分裂染色体上。更强烈束缚间期细胞通过前期,增加了一倍的紧密地绑定“固定分数。”在中期,然后变得更加简单末期。绑定的RUNX2交流更慢核仁比亚核的焦点,无论是否固定比例更大核仁。模型RUNX2的分子机制绑定在蛋白质编码不同核糖体RNA基因。RUNX2改变细胞通过有丝分裂亲和力增加染色体压缩,然后通过随后的减少有丝分裂阶段。RUNX2在有丝分裂染色体可能反映了它表观遗传在“书签”的目标函数基因在癌细胞。

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