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首页> 外文期刊>Journal of Cellular Physiology >Reprogramming the pluripotent cell cycle: restoration of an abbreviated G1 phase in human induced pluripotent stem (iPS) cells.
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Reprogramming the pluripotent cell cycle: restoration of an abbreviated G1 phase in human induced pluripotent stem (iPS) cells.

机译：重组多能细胞周期:恢复人类的缩写G1期诱导多能干细胞(iPS)细胞。

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摘要

Induced pluripotent stem (iPS) cells derived from terminally differentiated human fibroblasts are reprogrammed to possess stem cell like properties. However, the extent to which iPS cells exhibit unique properties of the human embryonic stem (hES) cell cycle remains to be established. hES cells are characterized by an abbreviated G1 phase ( approximately 2.5 h) and accelerated organization of subnuclear domains that mediate the assembly of regulatory machinery for histone gene expression [i.e., histone locus bodies (HLBs)]. We therefore examined cell cycle parameters of iPS cells in comparison to hES cells. Analysis of DNA synthesis [5-bromo-2'-deoxy-uridine (BrdU) incorporation], cell cycle distribution (FACS analysis and Ki67 staining) and subnuclear organization of HLBs [immunofluorescence microscopy and fluorescence in situ hybridization (FISH)] revealed that human iPS cells have a short G1 phase ( approximately 2.5 h) and an abbreviated cell cycle (16-18 h). Furthermore, HLBs are formed and reorganized rapidly after mitosis (within 1.5-2 h). Thus, reprogrammed iPS cells have cell cycle kinetics and dynamic subnuclear organization of regulatory machinery that are principal properties of pluripotent hES cells. Our findings support the concept that the abbreviated cell cycle of hES and iPS cells is functionally linked to pluripotency.
机译：诱导多能干细胞(iPS)细胞终末分化人类成纤维细胞拥有干细胞重新编程属性。人类的细胞表现出独特的属性胚胎干细胞细胞周期有待(他)建立。缩写G1期(约2.5小时)加速组织亚核的域协调管理机械的装配组蛋白的基因表达(即组蛋白位点身体(hlb)]。iPS细胞相比,他的参数细胞。(5-bromo-2 -deoxy-uridine (BrdU)合并),(流式细胞仪分析和Ki67细胞周期分布染色的hlb)和亚核的组织(免疫荧光显微镜和荧光原位杂交(FISH)]显示,人类“诱导多能性”细胞G1期较短(约2.5 h)和一个缩写细胞周期(16 - h)。此外,hlb形成和重组有丝分裂(在1.5 - 2小时)后迅速。因此,“诱导多能性”细胞重新编程细胞周期动力学和动态亚核的组织的监管机械的主要属性多能他细胞。他的缩写细胞周期的概念和“诱导多能性”细胞功能有关多能性。

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