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首页> 外文期刊>Journal of Cellular Physiology >Decrease in claudin-2 expression enhances cell migration in renal epithelial Madin-Darby canine kidney cells.
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Decrease in claudin-2 expression enhances cell migration in renal epithelial Madin-Darby canine kidney cells.

机译：减少claudin-2表达增强细胞迁移在肾上皮Madin-Darby犬肾脏细胞。

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摘要

Migration of renal epithelial cells increases after renal tubular damage, but its mechanism has not been clarified in detail. Hyperosmotic stress increased a cellular injury concomitant with a decrease in mRNA and protein expression of claudin-2 in renal tubular epithelial Madin-Darby canine kidney cells. We hypothesized that claudin-2 is involved in the regulation of cell migration. To knockdown claudin-2 expression, we made the cells expressing doxycycline-inducible claudin-2 shRNA vector. Claudin-2 knockdown affected neither the endogenous expression levels of claudin-1, -3, -4, and -7 nor the Triton X-100 solubility of these claudins. Transepithelial electrical resistance was increased by claudin-2 knockdown without affecting permeability to FITC-dextran (4,000 Da). BrdU incorporation assay and cell counting revealed that cell proliferation and viability are unaffected by claudin-2 knockdown. In the wound-healing assay, the recovery rate of wound area was increased by claudin-2 knockdown. The mRNA expression and activity of matrix metalloproteinase-9 (MMP-9) were increased by claudin-2 knockdown. A selective MMP-9 inhibitor suppressed cell migration in the claudin-2 knockdown cells. Hyperosmotic stress increased the expression and activity of MMP-9, which were inhibited by claudin-2 overexpression. These results suggest that the decrease in claudin-2 expression enhances cell migration mediated by the increase in the expression and activity of MMP-9.
机译：移植肾上皮细胞增加后肾小管损伤,但其机制没有详细澄清。增加细胞损伤相伴的在mRNA和蛋白表达减少在肾小管上皮Madin-Darby claudin-2犬肾细胞。claudin-2参与调节细胞迁移。使细胞表达doxycycline-inducibleclaudin-2 shRNA向量。无论是内源性表达水平的影响claudin-1, 3、4、7和海神x - 100这些claudins的溶解度。电阻被claudin-2增加击倒而不影响渗透率FITC-dextran (4000 Da)。细胞和细胞计数显示增殖和生存能力不受影响claudin-2击倒。伤口面积增加了的回收率claudin-2击倒。活动矩阵metalloproteinase-9 (MMP-9)是增加了claudin-2击倒。选择性MMP-9抑制剂抑制细胞claudin-2击倒细胞迁移。高渗的压力增加了表达和MMP-9的活动受到抑制claudin-2超表达。claudin-2表达下降增强细胞迁移的增加在MMP-9的表达和活性。

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