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首页> 外文期刊>Journal of Cellular Physiology >Molecular dissection of Otx1 functional domains in the zebrafish embryo.
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Molecular dissection of Otx1 functional domains in the zebrafish embryo.

机译：分子解剖Otx1功能域斑马鱼的胚胎。

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摘要

Otx proteins are involved in the induction of neurectoderm patterning and morphogenetic movements, leading to the formation of the vertebrate central nervous system. Despite lack of homology of sequence outside the homeodomain, a large body of evidence has shown that the Otx/Otd class of proteins has similar functions in many animal phyla. Thus, characterization of functional domains in proteins of this family would help in understanding how this functional equivalence operates. Our previous analysis using the zebrafish embryo (Bellipanni et al., 2000, Dev Biol 223:339-353), has suggested that induction of cell aggregation is a morphoregulatory role of Otx/Otd factors in embryonic development. We now use the induction of cell aggregation as an in vivo assay to examine the functional requirement for particular domains of the zOtx1 protein. We demonstrate that zOtx1 induces cell aggregation by acting as a transcriptional activator through its C-terminal region. Further, we show that a region of 37 amino acids in the C-terminal third of zOtx1 is necessary but not sufficient for this activation potential. The effects of selective deletion of each of the three homeodomain alpha-helices of zOtx1 on cell aggregation were also tested. Surprisingly, we find that helix 3, which is required for binding to DNA, is dispensable for stimulation of cell aggregation. Our results suggest that for transcriptional activation of at least one gene in the cell aggregation pathway, zOtx1 need not bind directly to DNA, but does require helix 1 and 2 of its homeodomain to interact with an as yet undefined DNA binding protein.
机译：第一项蛋白质参与的感应neurectoderm模式和形态形成运动,导致的形成脊椎动物的中枢神经系统。序列的同源性在homeodomain之外,大量的证据表明第一项/定单计划类的蛋白质也有类似的功能在许多动物类群。在蛋白质功能域的家庭将有助于了解这个功能吗等价。Dev 223:339 - 353)杂志,建议诱导细胞聚合是一个morphoregulatory第一/定单计划因素的作用胚胎发育。作为体内的细胞聚合试验检查特定的功能要求域的zOtx1蛋白质。作为zOtx1诱发细胞聚集通过其c端转录激活地区。氨基酸的c端第三zOtx1必要但不充分的激活的潜力。三种homeodomain阿尔法螺旋zOtx1细胞聚合也测试了。令人惊讶的是,我们发现螺旋3,绑定所需的DNA,是可有可无的刺激细胞聚合。建议的转录激活至少一个基因在细胞中聚合途径,zOtx1不需要直接绑定到DNA,但是需要homeodomain的螺旋1和2与一个还未定义的DNA结合蛋白质。

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