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首页> 外文期刊>Aquaculture International >Evaluation of the effects of probiotics in controlling bacterial necrosis symptoms in larvae of the mud crab Scylla serrata during mass seed production
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Evaluation of the effects of probiotics in controlling bacterial necrosis symptoms in larvae of the mud crab Scylla serrata during mass seed production

机译：益生菌在大规模生产种子蟹蟹Sylla serrata幼虫中控制细菌坏死症状的效果评估

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摘要

We aimed to evaluate the practicability of probiotics in controlling larval necrosis symptoms and mass mortality occurred during seed production of the mud crab Scylla serrata. We targeted a bacterium in the family Flavobacteriaceae that causes necrosis symptoms of mud crab larvae. Five candidate probiotic bacterial strains, which showed inhibitory effects against the causative bacterium but were innocuous to larvae, were screened from the seed production environment. Sterile culture medium supernatants of the candidate strains inhibited the causative bacterium through the secretion of extracellular factors. These inhibitory effects were largely reduced when the strains were inoculated into sea water, suggesting medium-dependent production of inhibitory factors. The strain that exhibited the highest inhibitory effect on larval necrosis in 7-day larval rearing experiments was selected as a probiotic strain. In large-scale larval rearing experiments, the probiotic treatment significantly suppressed larval necrosis symptoms and improved larval survival in the early rearing period until the fourth zoeal stage. However, the effect disappeared after the fifth (last) zoeal stage, corresponding to a decrease in inoculated probiotic bacterial counts in the larval rearing water. Almost all larvae had died by the first crab stage. The effectiveness of probiotics developed in vitro has not yet proved practical in commercial-scale seed production. The continuous proliferation of probiotic bacteria and extracellular production as inhibitory factors in larval rearing water are the key factors in developing probiotics for commercial-scale seed production.
机译：我们旨在评估益生菌在控制幼蟹坏死症状和泥蟹Scylla serrata种子生产过程中发生的大量死亡中的实用性。我们针对黄杆菌科中的一种细菌，该细菌引起泥蟹幼虫的坏死症状。从种子生产环境中筛选出五种候选益生菌菌株，它们对病原菌具有抑制作用，但对幼虫无害。候选菌株的无菌培养基上清液通过细胞外因子的分泌抑制病原菌。当将菌株接种到海水中时，这些抑制作用大大降低，表明抑制因子的培养基依赖性产生。选择在7天幼虫饲养实验中对幼虫坏死具有最高抑制作用的菌株作为益生菌菌株。在大规模的幼虫饲养实验中，益生菌治疗可显着抑制幼虫坏死症状，并在饲养的早期至第四黄牛期提高幼虫的存活率。然而，在第五个（最后一个）动物期后，这种作用消失了，这对应于幼体饲养水中接种的益生菌数量的减少。几乎所有的幼虫都在蟹的第一阶段死亡。尚未证明在体外开发的益生菌的有效性在商业规模的种子生产中是可行的。益生菌细菌的持续增殖和作为幼虫饲养水中抑制因子的细胞外生产是开发用于商业规模种子生产的益生菌的关键因素。

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