Chromosomal rearrangements during human epidermal keratinocyte differentiation.

Marella NV; Seifert B; Nagarajan P

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Chromosomal rearrangements during human epidermal keratinocyte differentiation.

机译：在人类表皮染色体重组角化细胞分化。

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摘要

Undifferentiated human epidermal keratinocytes are self-renewing stem cells that can be induced to undergo a program of differentiation by varying the calcium chloride concentration in the culture media. We utilize this model of cell differentiation and a 3D chromosome painting technique to document significant changes in the radial arrangement, morphology, and interchromosomal associations between the gene poor chromosome 18 and the gene rich chromosome 19 territories at discrete stages during keratinocyte differentiation. We suggest that changes observed in chromosomal territorial organization provides an architectural basis for genomic function during cell differentiation and provide further support for a chromosome territory code that contributes to gene expression at the global level.

机译：未分化的人类表皮角质化细胞可以诱导干细胞自我更新进行一个项目不同的分化氯化钙浓度的文化媒体。分化和3 d染色体涂染技术文档发生相当大的变化径向布置、形态学和染色体间基因之间的关联可怜的18号染色体和基因丰富的染色体19个地区在离散阶段角化细胞分化。变化观察到染色体的领土组织提供了一个架构的基础基因组在细胞分化和功能提供进一步支持染色体地区代码,导致基因表达式在全球层面。

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来源
《Journal of Cellular Physiology》 |2009年第1期|139-146|共8页
作者
Marella NV; Seifert B; Nagarajan P;
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作者单位

Department of Biological Sciences, University at Buffalo, State University of New York, Buffalo, New York 14260, USA.;

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原文格式 PDF
正文语种英语
中图分类细胞生物学;
关键词
Keratinocyte Differentiation; Cell Differentiation; Chromosome TerritoryChromosome rearrangementsgrowing mediaEpidermis;

机译：细胞角化细胞分化;染色体TerritoryChromosome分化;rearrangementsgrowing mediaEpidermis;

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Chromosomal rearrangements during human epidermal keratinocyte differentiation.

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