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首页> 外文期刊>Journal of Cellular Physiology >ERalpha and AP-1 interact in vivo with a specific sequence of the F promoter of the human ERalpha gene in osteoblasts.
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ERalpha and AP-1 interact in vivo with a specific sequence of the F promoter of the human ERalpha gene in osteoblasts.

机译：ERalpha和AP-1体内与特定的交互人类ERalpha F启动子序列在成骨细胞基因。

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摘要

Estrogen-responsive genes often have an estrogen response element (ERE) positioned next to activator protein-1 (AP-1) binding sites. Considering that the interaction between ERE and AP-1 elements has been described for the modulation of bone-specific genes, we investigated the 17-beta-estradiol responsiveness and the role of these cis-elements present in the F promoter of the human estrogen receptor alpha (ERalpha) gene. The F promoter, containing the sequence analyzed here, is one of the multiple promoters of the human ERalpha gene and is the only active promoter in bone tissue. Through electrophoretic mobility shift (EMSA), chromatin immunoprecipitation (ChIP), and re-ChIP assays, we investigated the binding of ERalpha and four members of the AP-1 family (c-Jun, c-fos, Fra-2, and ATF2) to a region located approximately 800 bp upstream of the transcriptional start site of exon F of the human ERalpha gene in SaOS-2 osteoblast-like cells. Reporter gene assay experiments in combination with DNA binding assays demonstrated that F promoter activity is under the control of upstream cis-acting elements which are recognized by specific combinations of ERalpha, c-Jun, c-fos, and ATF2 homo- and heterodimers. Moreover, ChIP and re-ChIP experiments showed that these nuclear factors bind the F promoter in vivo with a simultaneous occupancy stimulated by 17-beta-estradiol. Taken together, our findings support a model in which ERalpha/AP-1 complexes modulate F promoter activity under conditions of 17-beta-estradiol stimulation.
机译：过量往往有雌激素的基因反应(之前)定位下一个元素激活蛋白1 (AP-1)结合位点。考虑到之前和之间的交互被描述为AP-1元素调制特异性基因,我们调查了17-beta-estradiol响应能力和这些cis-elements礼物的作用F人类雌激素受体α的启动子(ERalpha)基因。序列分析,是多个推动者人类ERalpha基因的只有积极促进骨组织。电泳迁移率(EMSA)转变,染色质免疫沉淀反应(芯片)和re-ChIP化验,我们调查了绑定ERalpha和四个AP-1家族成员(c-Jun、c-fos Fra-2,和ATF2)地区位于约800英国石油公司上游的转录的开始F SaOS-2人类ERalpha基因的外显子osteoblast-like细胞。实验结合DNA结合分析表明,F子活动的控制下上游cis-acting元素哪些是被特定的组合ERalpha、c-Jun c-fos, ATF2 homo -形成。实验表明,这些核的因素绑定同时F子体内入住率17-beta-estradiol刺激。在一起,我们的研究结果支持模型ERalpha / AP-1复合物调制F子活动17-beta-estradiol的条件下刺激。

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