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首页> 外文期刊>Journal of Cellular Physiology >Changes in macrophage function and morphology due to biomedical polyurethane surfaces undergoing biodegradation.
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Changes in macrophage function and morphology due to biomedical polyurethane surfaces undergoing biodegradation.

机译：巨噬细胞功能和形态的变化生物医学聚氨酯表面发生生物降解。

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Monocytes are recruited to the material surface of an implanted biomedical device recognizing it as a foreign body. Differentiation into macrophages subsequently occurs followed by fusion to form foreign body giant cells (FBGCs). Consequently, implants can become degraded, cause chronic inflammation or become isolated by fibrous encapsulation. In this study, a relationship between material surface chemistry and the FBGC response was demonstrated by seeding mature monocyte-derived macrophages (MDMs) on polycarbonate-based polyurethanes that differed in their chemical structures (synthesized with poly(1,6-hexyl 1,2-ethyl carbonate) diol, and either (14)C-hexane diisocyanate and butanediol (BD) (referred to as HDI) or 4,4'-methylene bisphenyl diisocyanate and (14)C-BD (referred to as MDI)) and material degradation assessed. At 48 h of cell-material interaction, the FBGC attached to HDI were more multinucleated (73%) compared to MDI or the polystyrene (PS) control (21 and 36%, respectively). There was a fivefold increase in the synthesis and secretion of a protein with an approximate molecular weight of 48 kDa and a pI of 6.1 (determined by two-dimensional gel electrophoresis) only from cells seeded on HDI. Immunoprecipitation confirmed that MSE and CE were synthesized and secreted de novo. Immunoblotting also showed an increase in secreted monocyte-specific esterase (MSE) and cholesterol esterase (CE) from cells seeded on HDI relative to PS and MDI. Significantly more radiolabel ((14)C) release and esterase activity were elicited by MDMs on HDI than MDI (P < 0.05). The material that was more degradable (HDI), elicited greater protein synthesis and esterase secretion as well as more multinucleated MDMs than MDI, suggesting that the material surface chemistry modulates the function of MDM at the site of an inflammatory response to an implanted device.
机译：单核细胞被雇来的材料表面一个生物医学植入装置识别它一个异物。随后发生融合形成紧随其后异物巨细胞(FBGCs)。植入物可以成为退化,引起慢性由纤维炎症或变得孤立封装。材料表面化学和FBGC之间响应被播种成熟了monocyte-derived巨噬细胞(mdm)聚碳酸酯基聚氨酯,不同在他们的化学结构(合成聚碳酸1 6-hexyl 1 2-ethyl)二醇,和(14) C-hexane二异氰酸酯和丁二醇(BD)(称为人类发展指数)或4,4’亚甲基bisphenyl二异氰酸酯和(14)C-BD(指MDI))和材料退化评估。h(测量相互作用,FBGC附呈人类发展指数是更多的多核(73%)相比MDI或聚苯乙烯(PS)控制(21和36%,分别)。合成和分泌的一种蛋白质近似48 kDa的分子量和π6.1(由二维凝胶电泳)仅从细胞种子在人类发展指数。免疫沉淀反应证实MSE和CE是合成和分泌新创。免疫印迹也表现出增加分泌monocyte-specific酯酶(MSE)胆固醇酯酶(CE)从细胞播种人类发展指数相对于PS和MDI。radiolabel ((14) C)释放和酯酶的活动引起了mdm比MDI在人类发展指数(P < 0.05)。更多的可降解的材料(HDI),引起更大的蛋白质合成和酯酶分泌以及更多的多核mdm比MDI,表明材料表面化学MDM的调节功能植入的炎症反应设备。

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