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首页> 外文期刊>Journal of Cellular Physiology >Activity and expression of Xenopus laevis matrix metalloproteinases: Identification of a novel role for the hormone prolactin in regulating collagenolysis in both amphibians and mammals.
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Activity and expression of Xenopus laevis matrix metalloproteinases: Identification of a novel role for the hormone prolactin in regulating collagenolysis in both amphibians and mammals.

机译：活动和表达非洲爪蟾蜍光滑的矩阵金属蛋白酶:小说的识别催乳激素的调节作用collagenolysis两栖动物和哺乳动物。

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摘要

Prolactin (PRL) has long been implicated in Xenopus metamorphosis as an anti-metamorphic and/or juvenilizing hormone. Numerous studies showed that PRL could prevent effects of either endogenous or exogenous thyroid hormone (TH; T(3)). It has been shown that expression of matrix metalloproteinases (MMPs) is induced by TH during Xenopus metamorphosis. Direct in vivo evidence, however, for such anti-TH effects by PRL with respect to MMPs has not been available for the early phase of Xenopus development or metamorphosis. To understand the functional role of PRL, we investigated effects of PRL on Xenopus collagenase-3 (XCL3) and collagenase-4 (XCL4) expression in a cultured Xenopus laevis cell line, XL-177. Northern blot analysis demonstrated that XCL3 and XCL4 expression were not detected in control or T(3)-treated cells, but were differentially induced by PRL in a dose- and time-dependent fashion. Moreover, treatment with IL-1alpha as well as phorbol myristate acetate (PMA), a protein kinase C (PKC) activator, or H8, a protein kinase A (PKA) inhibitor, augmented PRL-induced collagenase expression, suggesting that multiple protein kinase pathways and cytokines may participate in PRL-induced collagenase expression. Interestingly, XCL3 expression could be induced in XL-177 cells by T(3), but only when co-cultured with prometamorphic Xenopus tadpole tails (stage 54/55), suggesting that the tails secrete a required intermediate signaling molecule(s) for T(3)-induced XCL3 expression. Taken together, these data demonstrate that XCL3 and XCL4 can be differentially induced by PRL and T(3) and further suggest that PRL is a candidate regulator of TH-independent collagenase expression during the organ/tissue remodeling which occurs in Xenopus development. J. Cell. Physiol. 201: 155-164, 2004. Copyright 2004 Wiley-Liss, Inc.
机译：催乳素(PRL)长期以来一直参与非洲爪蟾蜍作为anti-metamorphic蜕变和/或juvenilizing激素。表明,PRL可以预防的影响内源性或外源性甲状腺激素(TH);T(3))。基质金属蛋白酶(MMPs)是由TH在非洲爪蟾蜍蜕变。证据,然而,对于这种anti-TH效应PRL对基质金属蛋白酶没有可用的非洲爪蟾蜍的早期阶段或发展蜕变。PRL, PRL对非洲爪蟾蜍的影响调查collagenase-3 (XCL3)和collagenase-4 (XCL4)表现在培养非洲爪蟾蜍光滑的细胞线,xl - 177。没有检测到,XCL3 XCL4表达式在控制或T细胞(3)治疗,但是不同剂量和光杆载荷引起的按时间的时尚。十四烷酸IL-1alpha以及佛波醇酯(PMA)蛋白激酶C (PKC)的激活,或H8、一个蛋白激酶a (PKA)抑制剂,增强PRL-induced胶原酶表达,暗示多种蛋白激酶通路和细胞因子可能参与PRL-induced胶原酶表达。表达可以诱导xl - 177细胞T(3),但只有当培养prometamorphic非洲爪蟾蜍蝌蚪的尾巴(阶段54/55),这表明反面分泌需要中间信号分子(s)T(3)全身XCL3表达式。这些数据表明XCL3和XCL4可以不同诱导PRL和T (3)进一步表明,PRL调节器是一个候选人期间的TH-independent胶原酶表达发生在的器官/组织重构非洲爪蟾蜍的发展。155 - 164年,2004年。

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