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首页> 外文期刊>Journal of Cellular Physiology >Expression and tyrosine phosphorylation of Cbl regulates macrophage chemokinetic and chemotactic movement.
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Expression and tyrosine phosphorylation of Cbl regulates macrophage chemokinetic and chemotactic movement.

机译：Cbl的表达及酪氨酸磷酸化调节巨噬细胞chemokinetic和趋化现象的运动。

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Primary macrophages isolated from hck(-/-)fgr(-/-) mice display altered morphology and F-actin cytoskeletal structures and reduced migration. The ability of phorbol myristyl acetate (PMA), a protein kinase C activator that has been reported to increase macrophage spreading and carcinoma cell motility, to rescue these hck(-/-)fgr(-/-) defects was tested. Although PMA-treated wild-type and hck(-/-)fgr(-/-) macrophages exhibited a similar flattened, spread phenotype, PMA did not rescue the hck(-/-)fgr(-/-) macrophage migration defect. Instead, both PMA-treated wild type and hck(-/-)fgr(-/-) macrophages were defective in spontaneous and chemotactic migration and tyrosine phosphorylation of the Cbl protooncoprotein was decreased in both. Moreover, c-cbl(-/-) macrophages displayed the same impairment of motility as hck(-/-)fgr(-/-) macrophages and a similar morphology with less polarization and more dorsal ruffling than wild-type macrophages. As Hck and Fgr expression and activity were not decreased in c-cbl(-/-) macrophages, these results suggest that Cbl is likely to be an important downstream mediator of the Src family kinase-regulated macrophage motility pathway.
机译：主要巨噬细胞分离hck fgr (- / -) (- / -)老鼠显示形态学改变和f -肌动蛋白细胞骨架结构和减少迁移。佛波醇的能力十四烷基酯(PMA)蛋白激酶C的激活被报道增加巨噬细胞和癌扩散细胞活性,来拯救这些hck (- / -) fgr (- / -)缺陷进行了测试。野生型和hck (- / -) fgr(- / -)巨噬细胞表现出类似的夷为平地,表现型传播,PMA没有拯救hck fgr (- / -) (- / -)巨噬细胞迁移的缺陷。PMA-treated野生型和hck (- / -) fgr (- / -)巨噬细胞在自发和有缺陷的趋化现象的迁移和酪氨酸磷酸化的Cbl protooncoprotein在下降。巨噬细胞显示相同的障碍运动性hck (- / -) fgr(- / -)巨噬细胞和一个类似与极化和形态比野生型巨噬细胞背激怒。Hck和Fgr表达式和活动都没有减少c-cbl(- / -)巨噬细胞,这些结果表明,Cbl可能是一个重要的下游Src家族的中介kinase-regulated巨噬细胞活性途径。

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