Rap1, a small GTP-binding protein is upregulated during arrest of proliferation in human keratinocytes.

DSilva NJ; Mitra RS; Zhang ZKurnit DMBabcock CRPolverini PJCarey TE

首页> 外文期刊>Journal of Cellular Physiology >Rap1, a small GTP-binding protein is upregulated during arrest of proliferation in human keratinocytes.

【24h】

Rap1, a small GTP-binding protein is upregulated during arrest of proliferation in human keratinocytes.

机译：Rap1,一小GTP-binding蛋白质调节在人类逮捕扩散角化细胞。

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Rap1 is a small GTP-binding protein (SMG) that exists in two 95% homologous isoforms, rap1A and rap1B. The functions of the rap1 proteins are not well understood. In this report we examined expression and function of rap1 in primary (HOKs) and immortalized (IHOKs) human oral keratinocytes under different growth conditions. In HOKs, rap1 increased with passage number, suggesting a role in differentiation and arrest of proliferation. Similarly, when inhibition of proliferation and differentiation were induced in HOKs by 1.2 mM CaCl2, both rap1 and involucrin increased with increasing concentrations of CaCl2. However, when similar experiments were done with IHOKs, which continue to proliferate in the presence of 1.2 mM CaCl2, the increase in involucrin expression was similar to HOKs but there was no substantial increase in rap1, suggesting that increased expression of rap1 is linked to inhibition of proliferation rather than differentiation of keratinocytes. Upon transfection of immortalized keratinocyteswith rapGAP, which inactivates both isoforms of endogenous active rap1, enhanced proliferation was observed. Thus, we conclude that rap1 inhibits proliferation in keratinocytes.

机译：Rap1小GTP-binding蛋白质(SMG)存在于两个95%同源亚型,rap1A和rap1B。很好理解。表达和功能rap1在初级(hok)和人类口腔角质细胞无限增殖(IHOKs)在不同生长条件下。与通道数增加,暗示的作用在分化和增殖的逮捕。同样的,当扩散和抑制分化诱导hok的1.2毫米氯化钙,rap1和involucrin增加氯化钙的浓度增加。类似的实验用IHOKs,继续增殖的1.2毫米氯化钙,involucrin表达的增加类似于hok但没有实质性的rap1增加,表明增加rap1的表达与抑制而不是分化增殖角化细胞。keratinocyteswith rapGAP,使其失去活性亚型的内源性活性rap1增强观察扩散。rap1抑制增殖角化细胞。

著录项

来源
《Journal of Cellular Physiology》 |2003年第3期|532-540|共9页
作者
DSilva NJ; Mitra RS; Zhang ZKurnit DMBabcock CRPolverini PJCarey TE;
展开▼
作者单位

Department of Oral Medicine, Pathology and Oncology, University of Michigan School of Dentistry, Ann Arbor, Michigan 48109-1078, USA. njdsilva@umich.edu;

展开▼
收录信息
原文格式 PDF
正文语种英语
中图分类细胞生物学;
关键词
Keratinocytes; involucrin; Cell ProliferationIndividualized InstructionArrests;

机译：角化细胞;involucrin;细胞ProliferationIndividualized InstructionArrests;

相似文献

外文文献
中文文献
专利

1. Hepatitis B virus X protein promotes proliferation and upregulates TGF-β1 and CTGF in human hepatic stellate cell line, LX-2 [J] . Guang-Hui Guo, De-Ming Tan, Ping-An Zhu, 国际肝胆胰疾病杂志（英文版） . 2009,第001期
2. Bystin-like protein is upregulated in hepatocellular carcinoma and required for nucleologenesis in cancer cell proliferation [J] . Hanzhi Wang, Wei Xiao, Qinbo Zhou, 细胞研究：英文版 . 2009,第010期
3. Ectopic expression of neurotrophic peptide derived from saposin C increases proliferation and upregulates androgen receptor expression and transcriptional activity in human prostate cancer cells [J] . Yan Ding, Hui-Qing Yuan, Feng Kong, 亚洲男性学杂志：英文版 . 2007,第005期
4. Dahuang Zhechong Pill （大黄庶虫丸） Containing Serum Inhibited Platelet-Derived Growth Factor-Stimulated Vascular Smooth Muscle Cells Proliferation by Inducing G1 Arrest Partly via Suppressing Protein Kinase C α-Extracellular Regulated Kinase 1/2 Signaling Pathway [J] . 刘娜, 刘俊田, 姬媛媛, 中国结合医学杂志：英文版 . 2012,第005期
5. Antineoplastic Combined Chemotherapy Protocols; Apoptosis; Caspases; Catechin; Cell Line, Tumor; Ceramides; Drug Synergism; Enzyme Activation; Humans; Ibuprofen; Male; Mitogen-Activated Protein Kinases; Oxidative Stress; Phosphorylation; Prostatic Neoplasms; Proto-Oncogene Proteins c-bcl-2; Tumor Suppressor Protein p53;*脱噬作用; Caspase类; 儿茶素; 神经酰胺类; 药物协同作用; 酶激活; 布洛芬; 氧化性应激; 磷酰化; 前列腺肿瘤; 原致癌基因蛋白质 c-bcl-2 [J] . The Netherlands journal of medicine. . 2009,第6期

机译：Antineoplastic Combined Chemotherapy Protocols; Apoptosis; Caspases; Catechin; Cell Line, Tumor; Ceramides; Drug Synergism; Enzyme Activation; Humans; Ibuprofen; Male; Mitogen-Activated Protein Kinases; Oxidative Stress; Phosphorylation; Prostatic Neoplasms; Proto-Oncogene Proteins c-bcl-2; Tumor Suppressor Protein p53;*脱噬作用; Caspase类; 儿茶素; 神经酰胺类; 药物协同作用; 酶激活; 布洛芬; 氧化性应激; 磷酰化; 前列腺肿瘤; 原致癌基因蛋白质 c-bcl-2
6. Adu; Aged;; Aged, 80 and over;; Cadherins;; Carcinoma, Squamous Cell;; Cell Nucleus;; Cytoplasm;; Female;; Humans;; Laryngeal Neoplasms;; Male;; Middle Aged;; Neoplasm Staging;; Protein-Serine-Threonine Kinases;; Proto-Oncogene Proteins c-akt;; Retrospective Studies;; beta Catenin;; *成年人;; 老年人;; 老年人, 80以上;; 钙粘着糖蛋白类;; 癌, 鳞状细胞;; 细胞核;; 细胞质;; 喉肿瘤;; 肿瘤分期;; 蛋白质丝氨酸苏氨酸激酶;; 回顾性研究 [J] . Acta Anaesthesiologica Scandinavica . 2009,第10期

机译：Adu; Aged;; Aged, 80 and over;; Cadherins;; Carcinoma, Squamous Cell;; Cell Nucleus;; Cytoplasm;; Female;; Humans;; Laryngeal Neoplasms;; Male;; Middle Aged;; Neoplasm Staging;; Protein-Serine-Threonine Kinases;; Proto-Oncogene Proteins c-akt;; Retrospective Studies;; beta Catenin;; *成年人;; 老年人;; 老年人, 80以上;; 钙粘着糖蛋白类;; 癌, 鳞状细胞;; 细胞核;; 细胞质;; 喉肿瘤;; 肿瘤分期;; 蛋白质丝氨酸苏氨酸激酶;; 回顾性研究
7. Avian Reovirus Nonstructural Protein p17-Induced G(2)/M Cell Cycle Arrest and Host Cellular Protein Translation Shutoff Involve Activation of p53-Dependent Pathways [O] . Chulu, J.L.C., Huang, W.R., Wang, L., 2014

机译：avian Reovirus Nonstructural protein p17-Induced G(2)/m Cell Cycle arrest and Host Cellular protein Translation shutoff Involve activation of p53-Dependent pathways

Rap1, a small GTP-binding protein is upregulated during arrest of proliferation in human keratinocytes.

摘要

著录项

相似文献

相关主题

期刊订阅