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首页> 外文期刊>Journal of Cellular Physiology >Sonic hedgehog is involved in osteoblast differentiation by cooperating with BMP-2.
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Sonic hedgehog is involved in osteoblast differentiation by cooperating with BMP-2.

机译：声波刺猬成骨细胞通过配合BMP-2分化。

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摘要

The roles of Sonic hedgehog (Shh) and Bone morphogenetic protein-2 (Bmp-2) in osteoblast differentiation were investigated using in vitro cell systems. Recombinant amino-terminal portion of SHH (rSHH-N) dose dependently stimulated ALP activity in C3H10T1/2 and MC3T3-E1 cells. rSHH-N induced expression of Osteocalcin mRNA in C3H10T1/2 cells. A soluble form of the receptor for type IA BMP receptor antagonized rSHH-N-induced ALP activity in C3H10T1/2 and MC3T3-E1 cells, indicating that BMPs are involved in SHH-induced osteoblast differentiation. Simultaneous supplement with rSHH-N and BMP-2 synergistically induced ALP activity and expression of Osteocalcin mRNA in C3H10T1/2 cells. Pretreatment with rSHH-N for 6 h enhanced the response to BMP-2 by increasing ALP activity in C3H10T1/2 and MC3T3-E1 cells. Stimulatory effects of rSHH-N and additive effects with rSHH-N and BMP-2 on ALP activity were also observed in mouse primary osteoblastic cells. Transplantation of BMP-2 (1 microg) into muscle of mice induced formation of ectopic bone, whereas transplantation of r-SHH-N (1-5 microg) failed to generate it. These results indicate that Shh plays important roles in osteoblast differentiation by cooperating with BMP.
机译：音猬因子的角色(嘘)和骨头在成骨细胞形态形成protein-2 (Bmp-2)使用体外分化了细胞系统。嘘(rSHH-N)剂量依赖性刺激了高山活动C3H10T1/2和MC3T3-E1细胞。诱导骨钙素mRNA的表达C3H10T1/2细胞。IA型BMP引起受体rSHH-N-induced C3H10T1/2和高山活动MC3T3-E1细胞,这表明我国在SHH-induced成骨细胞分化。同时补充rSHH-N和BMP-2诱导高山活动和增效剂在C3H10T1/2骨钙素mRNA的表达细胞。通过增加高山BMP-2活动的响应在C3H10T1/2 MC3T3-E1细胞。rSHH-N和添加剂的效果的影响rSHH-N和BMP-2高山活动也老鼠主要的成骨细胞的细胞中观察到。移植BMP-2 (1 microg)到肌肉小鼠诱发异位骨形成,而移植r-SHH-N (1 - 5 microg)未能产生它。嘘,在成骨细胞中扮演重要角色分化与BMP合作。

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