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首页> 外文期刊>Journal of Cellular Physiology >Effect of cytokines on the proliferation/differentiation of stroma-initiating cells.
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Effect of cytokines on the proliferation/differentiation of stroma-initiating cells.

机译：细胞因子的影响增殖和分化的stroma-initiating细胞。

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A culture system that identifies the precursor of murine bone marrow fibroblastic stromal cells (stroma-initiating cells, SIC) has been developed. In this system, mature fibroblasts are depleted by adherence to plastic dishes and the nonadherent cells are seeded at a low density, which results in the formation of colonies composed of fibroblastic cells. Macrophage colony-stimulating factor (M-CSF) has been shown to accelerate the colony formation in the system. In this study, we examined the stroma-inducing activity of a number of cytokines. Neither granulocyte-CSF, stem cell factor, interleukin (IL)-1, IL-6, transforming growth factor, epidermal growth factor, insulin-like growth factor, platelet-derived growth factor, nor fibroblast growth factor showed the activity. Similarly, tumor necrosis factor (TNF) did not show any stroma-inducing activity, but the factor inhibited the stromal colony formation induced by M-CSF. In this study, we found that granulocyte/macrophage-CSF (GM-CSF) and IL-3, as well as M-CSF had the stroma-inducing activity. Neither an additive nor synergistic effect was observed when the three factors were assayed in various combinations. The stroma-inducing activity of M-CSF, GM-CSF and IL-3 was observed even if lineage-negative bone marrow cells were used as target cells, suggesting that mature hematopoietic cells such as macrophages and granulocytes were not involved in the induction of stromal colony formation by these factors. Our results raise the possibility that GM-CSF and IL-3 as well as M-CSF stimulate the proliferation or differentiation of the precursor of bone marrow fibroblastic stromal cells. Copyright 2000 Wiley-Liss, Inc.
机译：文化系统标识的前体小鼠骨髓成纤维细胞的间质细胞(stroma-initiating细胞,原文如此)发展。塑料盘子的依从性和枯竭不依从细胞的低密度,导致殖民地的形成成纤维细胞的细胞组成。集落刺激因子(csf)已被证明加快系统的集落形成。在这项研究中,我们调查了stroma-inducing许多细胞因子的活性。granulocyte-CSF、干细胞因子、白介素白介素(IL) 1日,转化生长因子,表皮生长因子、胰岛素样生长因素,血小板源生长因子,也没有纤维母细胞生长因子显示出活动。同样,肿瘤坏死因子(TNF)没有显示任何stroma-inducing活动,但因素抑制引起的基质集落形成csf。粒细胞/ macrophage-CSF (gm - csf)和IL-3以及- csf stroma-inducing活动。一种添加剂和协同效应观察到三个因素被化验各种组合。活动- csf, gm - csf和IL-3被观察到即使lineage-negative骨髓细胞作为靶细胞,这表明成熟如巨噬细胞和造血细胞粒细胞没有参与感应这些因素的基质集落形成。提高gm - csf的可能性和结果IL-3以及csf刺激增殖或骨的前体分化骨髓成纤维细胞的基质细胞。Wiley-Liss公司。

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