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首页> 外文期刊>Aquaculture Research >A spatiotemporal study of bacterial community profiles associated with Atlantic bluefin tuna larvae, Thunnus thynnus L., in three Mediterranean hatcheries
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A spatiotemporal study of bacterial community profiles associated with Atlantic bluefin tuna larvae, Thunnus thynnus L., in three Mediterranean hatcheries

机译：在三个地中海孵化场中与大西洋蓝鳍金枪鱼幼体Thunnus thynnus L.相关的细菌群落特征的时空研究

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During the first 2years of larval rearing trials with Atlantic bluefin tuna, survival was a challenging issue. As bacterial colonization of the gut has been shown to play a key role for other species, we studied the profiles of the microbiota associated with individual larvae at different stages in three distant hatcheries. The Bacterial Community Profile (BCP) was quantified based on PCR-DGGE analyses of partial amplicons from 16S rDNA. Considerable individual variability in BCP was observed before onset of feeding, and the BCP did not show regular fluctuation during ontogenesis. Microalgae were added to the rearing tanks in two of the three hatcheries, but it was not possible to distinguish the effect of location from the effect of algal addition on BCP. In one hatchery, the larvae were reared either with algal addition or in mesocosm, but due to high individual variability, no significant difference in BCP was detected between the two groups. It was hypothesized that this variability was caused by differences in health, physiological status and developmental stage of the larvae. A practical conclusion from the study is the need to analyse a considerable number of individuals to reflect statistically significant differences between the microbial communities associated with rearing groups.
机译：在大西洋蓝鳍金枪鱼幼体饲养试验的前两年中，生存是一个具有挑战性的问题。由于肠道细菌的定殖已被证明对其他物种也起着关键作用，因此我们研究了在三个遥远的孵化场不同阶段与单个幼体相关的微生物群的分布。基于16S rDNA的部分扩增子的PCR-DGGE分析，对细菌群落概况（BCP）进行了定量。在开始喂食之前，观察到BCP的个体差异很大，并且BCP在成瘤过程中没有规律的波动。在三个孵化场中的两个孵化场中，将微藻添加到饲养池中，但是无法将位置的影响与藻类添加对BCP的影响区分开。在一个孵化场中，幼体通过添加藻类或在中膜环境中进行饲养，但是由于个体变异性高，因此在两组之间未检测到BCP的显着差异。据推测，这种变异是由幼虫的健康，生理状况和发育阶段的差异引起的。该研究的一个实际结论是需要分析大量个体以反映与饲养组相关的微生物群落之间的统计学显着差异。

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