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首页> 外文期刊>Journal of Cellular Physiology >Suppression of transforming growth factor-beta isoforms, TGF-beta receptor type II, and myofibroblast differentiation in cultured human corneal and limbal fibroblasts by amniotic membrane matrix.
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Suppression of transforming growth factor-beta isoforms, TGF-beta receptor type II, and myofibroblast differentiation in cultured human corneal and limbal fibroblasts by amniotic membrane matrix.

机译：转化生长因子的抑制亚型、鉴定及受体II型myofibroblast分化培养的人类和角膜缘的羊膜成纤维细胞膜矩阵。

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摘要

Down-regulation of the transforming growth factor-beta (TGF-beta) signaling system is a strategy for preventing scarring during wound healing. Human corneal and limbal fibroblasts were cultured on the stromal matrix side of preserved human amniotic membrane. The levels of TGF-beta1, beta2, and beta3 and TGF-beta type II receptor transcripts and TGF-beta1 and beta2 proteins were suppressed as early as 8 hr and more dramatically at 24 hr after contact with an amniotic membrane. This suppressive effect was accompanied by down-regulation of alpha-smooth muscle actin, EDA spliced form of fibronectin, and integrin alpha5. It persisted even when challenged by 10 ng/ml TGF-beta1. In contrast with their counterparts grown on plastic or in collagen gel, such suppression in amniotic membrane cultures remained complete after 1 week of culturing. Cells cultured on amniotic membrane showed significantly reduced [3H]-thymidine incorporation compared to cells cultured on plastic and displayed no DNA fragmentation. These results reveal a novel mechanism by which the TGF-beta signaling system, DNA synthesis, and subsequent myofibroblast differentiation can be suppressed by an amnionic membrane matrix. This action explains in part the antiscarring results of amniotic membrane transplantation used for ocular surface reconstruction, a surgical technique applicable to other subspecialties. It may also explain in part why fetal wound healing is scarless.
机译：的下调将增长因子及信号系统是一个在伤口策略防止疤痕愈合。培养基质矩阵的一面吗保存人羊膜。TGF-beta1、beta2 beta3和鉴定及II型受体转录和TGF-beta1 beta2蛋白质抑制早在8小时,与一个更为戏剧性的在24小时后联系羊膜。伴随着alpha-smooth下调肌肉肌动蛋白、纤连蛋白的EDA拼接形式,和整合素alpha5。受到10 ng / ml TGF-beta1挑战。与他们的同行在塑料或增长胶原蛋白凝胶,这种抑制羊膜1周后膜文化仍然是完整的的培养。显示显著降低[3 h]胸苷公司相比,细胞培养塑料和显示没有DNA碎片。结果揭示了一个新颖的机制及信号系统、DNA合成和随后myofibroblast分化抑制由羊膜的膜矩阵。行动部分antiscarring结果解释道羊膜移植眼表面重建手术技术适用于其他细分专业。也可以部分解释为什么胎儿伤口愈合无疤。

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