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首页> 外文期刊>Journal of Cellular Physiology >Increased expression of the distal, but not of the proximal, Gata1 transcripts during differentiation of primary erythroid cells.
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Increased expression of the distal, but not of the proximal, Gata1 transcripts during differentiation of primary erythroid cells.

机译：提高表达的远端,但不是的近端,Gata1期间成绩单原发性红细胞分化细胞。

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摘要

Gata1 is expressed from either one of two alternative promoters, the erythroid (proximal to the AUG) and the testis (distal to the AUG) promoter, both used by hemopoietic cells. To clarify the role of the distal and proximal Gata1 transcripts in erythroid differentiation, we determined by specific reverse transcriptase-polymerase chain reactions their relative levels of expression during the differentiation of erythroid precursors purified from the spleen of mice treated with phenylhydrazine (PHZ) or infected with the anemia-inducing strain of the Friend virus (FVA cells). PHZ cells are erythroid precursors that progress in vivo to erythroblasts in 3 days. Both PHZ and FVA cells synchronously proliferate and differentiate in vitro in the presence of erythropoietin (EPO). The levels of total and of distal, but not of proximal, Gata1 transcripts increased by five- to eightfold during in vivo and in vitro differentiation of FVA and PHZ cells. The increase in expression was temporally associated with an increase in the expression of Eklf, Scl, and Nfe2, three genes required for erythroid differentiation, and preceded by 24 h the repression of Gata2 and Myb expression. The day 1 PHZ cells that survived 18 h in the absence of EPO do not express globin genes and express detectable levels of distal but not of proximal Gata1 transcripts. These cells activate the expression of the globin genes within 2 h when exposed to EPO. Therefore, during erythroid differentiation of primary cells, increased expression of distal Gata1 transcripts underlies the increase in the expression of total Gata1 associated with the establishment of the erythroid differentiation program.
机译：Gata1表示从两种替代促进剂,红细胞(近端8月)和睾丸8月(远端)子,所使用的造血细胞。澄清的作用远端和近端Gata1成绩单在红细胞分化,我们由特定的反向transcriptase-polymerase链式反应的相对水平的表达红细胞前体细胞的分化净化从小鼠的脾脏苯肼(PHZ)或感染朋友的病毒(FVA anemia-inducing压力细胞)。在3天进步体内成红血球细胞。PHZ同步和FVA细胞增殖区分体外的存在促红细胞生成素(EPO)。近端远端,但不是,Gata1记录在体内增加了5 - 8倍和体外分化FVA PHZ细胞。与增加的表达有关Eklf、sci和Nfe2,所需的三个基因红细胞分化,之前24小时Gata2的镇压和Myb表达式。第一天PHZ细胞存活18 h在没有促红细胞生成素不表达球蛋白基因和表达可检测水平远但不近端Gata1记录。在2 h当球蛋白基因的表达促红细胞生成素。分化的主要细胞,增加远端Gata1成绩单背后的表情增加总Gata1的表达与建立联系在一起红细胞分化程序。

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