...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Cellular Physiology >Successive formative stages of precartilaginous mesenchymal condensations in vitro: modulation of cell adhesion by Wnt-7A and BMP-2.
【24h】

Successive formative stages of precartilaginous mesenchymal condensations in vitro: modulation of cell adhesion by Wnt-7A and BMP-2.

机译：连续precartilaginous的形成阶段间叶细胞进行体外:调制的细胞粘附Wnt-7A和BMP-2。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

High-density chick limb bud cell culture is a useful model to study mesenchymal condensatifons and chondrogenesis. Most previous studies have focused on the effects of soluble reagents on terminal chondrogenic differentiation and have not defined the early cellular processes and signaling events. In this study, we defined five successive stages in the differentiation process: 1) dissociated cells, 2) small aggregates, 3) formation of cell clusters, 4) precartilaginous condensations, and 5) cartilage nodule. We used RCAS retrovirus-mediated Wnt-7a gene transduction to test the effect of Wnt-7a on the differentiation process. We found that Wnt-7a suppressed chondrogenic differentiation. Wnt-7a did not inhibit the initiation of condensation formation but blocked the progression of precartilaginous condensations to cartilage nodules. The Wnt-7a-transduced cultures showed characteristics of a less mature culture with persistent expression of NCAM, N-cadherin, wider distribution of integrin beta1 and fibronectin, and suppression of tenascin-C. BMP-2 is known to enhance chondrogenic differentiation in these cultures by promoting cell clusters to form continuous sheet-like precartilaginous condensations. However, cultures exposed to both BMP-2 and Wnt-7a showed inhibition of chondrogenic differentiation. Different signaling molecules such as Wnt-7a and BMP-2 may have antagonistic effects on cartilage differentiation and the gradient of the two molecules may be involved in defining the boundaries of the initial precartilaginous condensation. We propose that the shape of the precartilaginous condensations may be modulated by local concentrations of signaling molecules, such as Wnt-7a and BMP-2, which act to alter cell-substrate and cell-cell adhesions.
机译：高密度小鸡肢芽细胞培养是a研究间充质condensatifons有用的模型和软骨形成。专注于可溶性试剂的影响终端chondrogenic分化和没有定义早期的细胞过程信号事件。在分化过程中连续的阶段:1)分离细胞,2)总量小,3)细胞集群的形成,4)precartilaginous密集的,5)软骨结节。rca retrovirus-mediated Wnt-7a基因转导Wnt-7a的测试效果分化的过程。抑制chondrogenic分化。没有抑制凝结的开始吗形成但阻止病情发展precartilaginous冷凝,软骨结节。一个不太成熟的文化的特征持久的NCAM的表达,N-cadherin,更广泛整合素beta1、纤粘连蛋白的分布,和tenascin-C的抑制。加强在这些chondrogenic分化文化通过促进细胞集群形成连续片状precartilaginous冷凝。BMP-2和Wnt-7a显示抑制chondrogenic分化。分子Wnt-7a和BMP-2等对抗对软骨分化的影响和两个分子可能的梯度参与定义的边界初始precartilaginous凝结。precartilaginous的形状密集的可能被当地的调制信号分子的浓度,如Wnt-7a BMP-2,改变行动细胞基质,和粘连。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号