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Bacteria in shrimp pond sediments: their role in mineralizing nutrientsand some suggested sampling strategies

机译:虾塘沉积物中的细菌:其在矿化营养物中的作用和一些建议的采样策略

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摘要

Strategies for sampling sediment bacteria were examined in intensive shrimp, Penaeus monodon (Fabricius), ponds in tropical Australia. Stratified sampling of bacteria at the end of the production season showed that the pond centre, containing flocculated sludge, had significantly higher bacterial counts (15.5 X 10(9) g(-1) dw) than the pond periphery (8.1 X 10(9) g(-1) dw), where the action of aerators had swept the pond floor. The variation in bacterial counts between these two zones within a pond was higher than that between sites within each zone or between ponds. Therefore, sampling effort should be focused within these zones: for example, sampling two ponds at six locations within each of the two zones resulted in a coefficient of variation of approximate to 5%. Bacterial numbers in the sediment were highly correlated with sediment grain size, probably because eroded soil particles and organic waste both accumulated in the centre of the pond. Despite high inputs of organic matter added to the ponds, principally as pelleted feeds, the mean bacterial numbers and nutrient concentrations (i.e. organic carbon, nitrogen and phosphorus) in the sediment were similar to those found in mangrove sediments. This suggests that bacteria are rapidly remineralizing particulates into soluble compounds. Bacterial numbers were highly correlated with organic carbon and total kjeldahl nitrogen in the sediment, suggesting that these were limiting factors to bacterial growth.
机译:在热带澳大利亚的集约化虾,斑节对虾(Fabricius)的池塘中研究了沉积物细菌采样的策略。在生产季节结束时对细菌进行分层采样显示,包含絮凝污泥的池塘中心的细菌数(15.5 X 10(9)g(-1)dw)明显高于池塘外围(8.1 X 10(9) )g(-1)dw),其中曝气器的作用已席卷池塘底。池塘中这两个区域之间细菌计数的变化高于每个区域内或池塘之间的细菌计数的变化。因此,采样工作应集中在这些区域内:例如,在两个区域中每个区域的六个位置对两个池塘进行采样,得出的变异系数约为5%。沉积物中的细菌数量与沉积物的粒径高度相关,这可能是因为侵蚀的土壤颗粒和有机废物都聚集在池塘中心。尽管向池塘中添加了大量有机物,主要是颗粒饲料,但沉积物中的平均细菌数和营养物浓度(即有机碳,氮和磷)与红树林沉积物中的相似。这表明细菌正在迅速将矿物质再矿化成可溶性化合物。细菌数量与沉积物中的有机碳和凯氏定氮总量高度相关,表明这些是细菌生长的限制因素。

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