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首页> 外文期刊>Journal of Cellular Physiology >Differential regulation of keratinocyte growth factor and hepatocyte growth factor/scatter factor by different cytokines in human corneal and limbal fibroblasts.
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Differential regulation of keratinocyte growth factor and hepatocyte growth factor/scatter factor by different cytokines in human corneal and limbal fibroblasts.

机译：微分调节角质细胞生长因子和肝细胞生长因子/散射由不同的细胞因子在人类角膜的因素和缘的成纤维细胞。

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摘要

Corneal epithelial stem cells and transient amplifying cells are located in the limbal and corneal regions, respectively. In a serum-free medium with or without different cytokines, limbal fibroblasts consistently produced greater levels of keratinocyte growth factor (KGF) transcript and protein than corneal fibroblasts, whereas corneal fibroblasts produced greater levels of hepatocyte growth factor/ scatter factor (HGF/SF) transcript and protein than limbal fibroblasts. Expression of HGF/SF transcript and protein was up-regulated mildly by epidermal growth factor (EGF), transforming growth factor-alpha (TGF-alpha), or platelet-derived growth factor B (PDGF-BB) but markedly by interleukin-1 beta (IL-1 beta) and was more pronounced in limbal than in corneal fibroblasts. Expression of KGF transcript was down-regulated by EGF, TGF-alpha, and PDGF-BB, was markedly up-regulated by IL-1 beta, and was more pronounced in limbal than in corneal fibroblasts. Expression of KGF protein was up-regulated markedly byIL-1 beta and moderately by PDGF-BB, especially in limbal fibroblasts. TGF-beta 1 uniquely turned off transcript and protein expression of HGF/SF and KGF in corneal fibroblasts. Although its transcript levels were similarly down-regulated in limbal fibroblasts, KGF protein levels were paradoxically up-regulated by TGF-beta 1 when added alone or with TGF-alpha or IL-1 beta. These data indicate that KGF and HGF/SF, two fibroblast-derived epithelial mitogens, are expressed differentially by limbal and corneal fibroblasts and are modulated by cytokines activated during epithelial-mesenchymal interactions, suggesting that they may play a different role in modulating corneal epithelial stem cells and transient amplifying cells.
机译：角膜上皮干细胞和瞬态放大细胞位于缘的角膜地区,分别。中有或没有不同的细胞因子,缘的成纤维细胞产生更大角质细胞生长因子(KGF)水平比角膜成纤维细胞转录和蛋白质,而角膜成纤维细胞产生更大肝细胞生长因子水平/散射因子(HGF / SF)转录和蛋白质缘的成纤维细胞。转录和蛋白质差异温和了表皮生长因子(EGF)、转换生长因子-α(是),或血小板源生长因子B (PDGF-BB)但是由ββ(il - 1)及明显更明显比在角膜缘的吗成纤维细胞。抑制表皮生长因子,是PDGF-BB,表达显著上调了il - 1β,在比角膜缘的成纤维细胞。差异显著byIL-1β和适度PDGF-BB,尤其是在缘的成纤维细胞。及1成绩单和独特蛋白质的表达HGF / SF和KGF在角膜成纤维细胞。类似的抑制在缘的成纤维细胞,KGF蛋白质含量是矛盾的差异及1当单独或补充道是和il - 1β。两个fibroblast-derived KGF和HGF / SF上皮有丝分裂原,表达不同缘的,角膜成纤维细胞和在调节细胞因子的激活epithelial-mesenchymal交互,暗示他们可能在调节发挥不同的作用角膜上皮干细胞和瞬态放大细胞。

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