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首页> 外文期刊>Journal of Cellular Physiology >Uncoordinated, transient mosaic patterns of intestinal hydrolase expression in differentiating human enterocytes.
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Uncoordinated, transient mosaic patterns of intestinal hydrolase expression in differentiating human enterocytes.

机译：不协调,瞬态的马赛克模式肠道水解酶表达人类肠上皮细胞分化。

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摘要

The heterogenous expression of brush border membrane hydrolases by the human enterocyte-like Caco-2 cell line during morphological and functional differentiation in vitro was investigated at the cellular level. Indirect immunofluorescence revealed that the heterogeneous ("mosaic") expression of sucrase-isomaltase, lactase, aminopeptidase N, and alkaline phosphatase was, in fact, transient in nature. The labeling indexes for each hydrolase gradually increased during culture at postconfluence in order to reach a maximum (> or = 90%) after 30 days, concomitant with an upregulation of their respective protein expression levels. In contrast, dipeptidylpeptidase IV labeling remained relatively constant. Backscattered electron imaging analysis in midstage (12 days postconfluence) monolayers demonstrated a lack of correlation between brush border membrane development and expression of each enzyme studied. Moreover, double immunostaining revealed that none of the other four hydrolases correlated directly with sucrase-isomaltase expression. Finally, immunodetection for the proliferation-associated antigen KI-67 revealed a transient mosaic pattern of proliferation which was inversely related to Caco-2 cell differentiation. These data indicate that enterocytic differentiation-related (as well as proliferation-related) gene expression in Caco-2 cells is regulated but uncoordinated at the cellular level, suggesting that an overall control mechanism is lacking.
机译：刷状缘的异构表达式由人类enterocyte-like膜水解酶在形态和Caco-2细胞系体外功能分化在细胞水平上研究。免疫荧光显示异构(“马赛克”)的表达sucrase-isomaltase、乳糖酶、氨基肽酶N,和碱性磷酸酶,事实上,转瞬即逝在自然界中。水解酶在文化逐渐增加postconfluence以达到最大(>或= 90%)后30天,相伴的upregulation各自的蛋白质表达水平。第四dipeptidylpeptidase标签仍相对恒定的。成像分析midstage(12天postconfluence)层表现出缺乏刷状缘膜之间的相关性每个酶的开发和表达式研究。没有其他的四个水解酶相关的直接与sucrase-isomaltase表达式。最后,immunodetection的proliferation-associated抗原ki - 67显示瞬态马赛克的扩散模式Caco-2细胞呈负相关分化。enterocytic differentiation-related(以及在Caco-2扩散)基因表达细胞是监管但不协调细胞水平,这表明一个整体缺乏控制机制。

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