...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Cellular Physiology >Cytoplasmic juxtamembrane domain of the human EGF receptor is required for basolateral localization in MDCK cells.
【24h】

Cytoplasmic juxtamembrane domain of the human EGF receptor is required for basolateral localization in MDCK cells.

机译：胞质近膜域中的人类表皮生长因子基底受体需要本地化在MDCK细胞。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Although it is well established that epidermal growth factor receptors (EGFRs) are asymmetrically expressed at the basolateral plasma membrane in polarized epithelial cells, how this process is regulated is not known. The purpose of this study was to address the mechanism of directed EGFR basolateral sorting using the Madin-Darby canine kidney (MDCK) cell model. The first set of experiments established sorting patterns for endogenous canine EGFRs. The polarity of the canine EGFR was not quantitatively affected by differences in electrical resistance exhibited by the MDCK I and MDCK II cell strains. In both cases, greater than 90% of total surface EGFRs was localized to the basolateral surface. Canine EGFRs sort directly to the basolateral membrane from the trans-Golgi network with a half-time of approximately 45 min and have an approximate t1/2 of 12.5 h once reaching the basolateral surface. Human holoreceptors expressed in stably transfected MDCK cells also localize to the basolateral membrane with similar efficiency. To identify EGFR sequences necessary for basolateral sorting, MDCK cells were transfected with cDNAs coding for cytoplasmically truncated human receptor proteins. Human EGFRs truncated at Arg-651 were localized predominantly at the apical surface of filter-grown cells, whereas receptors truncated at Leu-723 were predominantly basolateral. These results suggest that the cytoplasmic juxtamembrane domain contains a positive basolateral sorting determinant. Moreover, the EGFR ectodomain or transmembrane domain may possess a cryptic sequence that specifically interacts with the apical sorting machinery once the dominant basolateral sorting signal is removed. Further elucidation of the precise location of these signals will enhance our basic understanding of regulated plasma membrane sorting, as well as the functional consequences of inappropriate EGFR expression associated with certain pathophysiologic and malignant states.
机译：虽然证实表皮生长因子受体(举)不对称地表达了在基底外侧质膜极化上皮细胞,这个过程是如何监管尚不清楚。本研究的目的是解决基底的定向机制EGFR排序使用Madin-Darby狗肾细胞(MDCK)模型。内生犬EGFRs排序模式。极性的犬类EGFR不是定量影响的差异电阻MDCK我和展出第二MDCK细胞压力。90%的总表面EGFRs本地化基底表面。从trans-Golgi基底外侧膜网络的半场大约45分钟一次近似t1/2 12.5 h到达基底外侧表面。holoreceptors表达稳定转染MDCK细胞也本地化基底外侧膜与效率。表皮生长因子受体基底序列所需的排序,MDCK细胞转染的互补编码cytoplasmically截断人类受体蛋白质。本地化主要在顶端表面filter-grown细胞上,而受体截断亮氨酸- 723主要是基底。结果表明,胞质近膜域中包含了一个积极的管基底排序行列式。表皮生长因子受体ectodomain或跨膜域拥有专门的序列与顶端排序机制基底主要排序信号移除。这些信号将增强我们基本的位置对监管的质膜的理解排序,以及功能的后果不适当的表皮生长因子受体表达有关某些病理生理的和恶性状态。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号